Zeng Qiao, Xiao Jian-Hua, Wan Zhi-Gang, Zhang Yu-Kuai, Liu Chuan-Ai, Yang Qiu-Lin
Institute of Pathogenic Biology, Nanhua University, Hengyang 421001, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Apr 30;23(2):86-9.
To acquire and analyze adult stage Schistosoma japonicum (Chinese strain) expressed sequence tags and new genes from an adult S. japonicum cDNA library, and to search new vaccine candidates and drug targets.
A cDNA library was constructed from adult stage S. japonicum. Clones were selected randomly from the cDNA library and were sequenced. ESTs and new genes were acquired after analysis in GenBank databases by BLAST and other programs. All ESTs and new genes were submitted to GenBank and received accession numbers.
149 ESTs were acquired from a total 382 clones that were randomly selected from the adult S. japonicum cDNA library. All ESTs were successfully submitted to the dbEST at Genbank. Some of them were homologous with sequences of male, female, egg, schistosomula, cercaria and miracidia of S. japonicum. 18 new genes of adult S. japonicum were acquired. Some genes were housekeeping genes and some genes might be interesting as vaccine candidates or drugs targets.
The EST strategy is a rapid, efficient and economical method to acquire ESTs and to discover new genes of adult stage S. japonicum from cDNA libraries.
从日本血吸虫（中国株）成虫cDNA文库中获取并分析成虫期表达序列标签及新基因，寻找新的疫苗候选物和药物靶点。
构建日本血吸虫成虫cDNA文库。从cDNA文库中随机挑选克隆进行测序。通过BLAST等程序在GenBank数据库中分析后获得EST和新基因。所有EST和新基因均提交至GenBank并获得登录号。
从日本血吸虫成虫cDNA文库中随机挑选的382个克隆中共获得149个EST。所有EST均成功提交至Genbank的dbEST。其中一些与日本血吸虫雄虫、雌虫、虫卵、童虫、尾蚴和毛蚴的序列同源。获得了18个日本血吸虫成虫新基因。一些基因是管家基因，一些基因可能作为疫苗候选物或药物靶点具有研究价值。
EST策略是从cDNA文库中获取日本血吸虫成虫EST和发现新基因的快速、高效且经济的方法。
