Zhang Rui-juan, Zhu Huai-min, Li Xiang-yu
Department of Etiological Biology, Second Military Medical University, Shanghai 200433, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2004 Apr 30;22(2):83-5.
To develop and identify the monoclonal antibodies (McAbs) against Region II+ motif in circumsporozoite protein of Plasmodium falciparum.
BALB/c mice were immunized with 12 peptides within Region II+ in circumsporozoite protein of P. falciparum. Spleen cells isolated from the immunized mice were fused with myeloma cell. After three times screening with ELISA, 3 positive hybridoma cell lines were obtained.
ELISA test indicated that the McAbs reacted with recombinant circumsporozoite protein fragment containing tandemly repeat region and conserved Region II+. IFA test showed that the McAbs recognized not only the sporozoites of P. falciparum, but also the sporozoifes of P. yoelii.
McAbs obtained can probe the Region II+ motif in circumsporozoite protein of P. falciparum, which might also recognize that of other Plasmodium species.
制备并鉴定抗恶性疟原虫环子孢子蛋白II +区域基序的单克隆抗体（McAbs）。
用恶性疟原虫环子孢子蛋白II +区域内的12种肽免疫BALB / c小鼠。从免疫小鼠中分离的脾细胞与骨髓瘤细胞融合。经ELISA三次筛选，获得3株阳性杂交瘤细胞系。
ELISA试验表明，这些单克隆抗体与含有串联重复区域和保守II +区域的重组环子孢子蛋白片段反应。间接荧光抗体试验（IFA）表明，这些单克隆抗体不仅识别恶性疟原虫的子孢子，也识别约氏疟原虫的子孢子。
获得的单克隆抗体可探测恶性疟原虫环子孢子蛋白中的II +区域基序，也可能识别其他疟原虫属的该基序。
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