Sina B J, Wright C, Atkinson C T, Ballou R, Aikawa M, Hollingdale M
Biomedical Research Institute, Rockville, MD, USA.
Mol Biochem Parasitol. 1995 Feb;69(2):239-46. doi: 10.1016/0166-6851(94)00198-v.
Previous studies demonstrated that immunization with Plasmodium falciparum sporozoites protected mice against Plasmodium berghei sporozoite infection and that this cross-protection was mediated, at least in part, by anti-sporozoite antibody. The experiments presented in this report show that serum and monoclonal antibodies derived from these protected mice identify a novel 42/54-kDa antigen (designated Circumsporozoite Protein 2 or CSP-2) in both P. falciparum and P. berghei sporozoites. Anti-CSP-2 monoclonal antibody blocks invasion of P. falciparum and P. berghei sporozoites into hepatoma cells in vitro and binds the cell surface of sporozoites. Passive transfer of anti-CSP-2 monoclonal antibody protected mice from P. berghei sporozoite infection. Therefore, CSP-2 appears to play a role in the cross-protective immune response observed.
先前的研究表明，用恶性疟原虫子孢子免疫可保护小鼠免受伯氏疟原虫子孢子感染，且这种交叉保护至少部分是由抗子孢子抗体介导的。本报告中的实验表明，来自这些受保护小鼠的血清和单克隆抗体可识别恶性疟原虫和伯氏疟原虫子孢子中的一种新的42/54-kDa抗原（命名为环子孢子蛋白2或CSP-2）。抗CSP-2单克隆抗体在体外可阻断恶性疟原虫和伯氏疟原虫子孢子侵入肝癌细胞，并与子孢子的细胞表面结合。抗CSP-2单克隆抗体的被动转移可保护小鼠免受伯氏疟原虫子孢子感染。因此，CSP-2似乎在观察到的交叉保护性免疫反应中发挥作用。
