Rocha E M, Hollingdale M R, Sina B, Leland P, Lopes J D, Krettli A U
Department of Parasitology, ICB, Federal University of Minas Gerais, Brazil.
J Eukaryot Microbiol. 1993 Jan-Feb;40(1):61-3. doi: 10.1111/j.1550-7408.1993.tb04882.x.
Monoclonal antibodies that react with the circumsporozoite protein of the avian malaria Plasmodium gallinaceum sporozoites also reacted with circumsporozoite protein of the rodent malaria Plasmodium berghei. Two types of reactivity were identified: 1) two monoclonal antibodies reacted with P. berghei sporozoite protein by enzyme-linked immunosorbent assay, Western blot and indirect immunofluorescence antibody, 2) six other monoclonal antibodies reacted with P. berghei sporozoites by ELISA and Western blot only. We studied whether these differences could be explained by reactivity in enzyme-linked immunosorbent assay with different P. berghei circumsporozoite peptides. Although all P. gallinaceum monoclonal antibodies reacted with the P. berghei repeats, the first group reacted with a conserved peptide sequence, N1, whereas the second group did not. These results suggest that circumsporozoite proteins from P. gallinaceum and P. berghei share common epitopes. The biological significance of our finding is not yet clear. Indeed, the cross-reactive monoclonal antibodies giving a positive indirect immunofluorescence antibody with the P. berghei sporozoites only caused a borderline effect on the living P. berghei parasites in vitro as measured by inhibition of sporozoite infectivity.
与鸡疟原虫疟原虫子孢子环子孢子蛋白发生反应的单克隆抗体，也与伯氏疟原虫的环子孢子蛋白发生反应。鉴定出两种反应类型：1）两种单克隆抗体通过酶联免疫吸附测定、蛋白质印迹法和间接免疫荧光抗体法与伯氏疟原虫子孢子蛋白发生反应；2）另外六种单克隆抗体仅通过酶联免疫吸附测定和蛋白质印迹法与伯氏疟原虫子孢子发生反应。我们研究了这些差异是否可以通过酶联免疫吸附测定中与不同伯氏疟原虫环子孢子肽的反应性来解释。虽然所有鸡疟原虫单克隆抗体都与伯氏疟原虫重复序列发生反应，但第一组与保守肽序列N1发生反应，而第二组则不反应。这些结果表明，鸡疟原虫和伯氏疟原虫的环子孢子蛋白具有共同的表位。我们这一发现的生物学意义尚不清楚。实际上，与伯氏疟原虫子孢子产生阳性间接免疫荧光抗体的交叉反应性单克隆抗体，通过抑制子孢子感染性测定，在体外对活的伯氏疟原虫寄生虫仅产生了临界效应。
