[日本血吸虫胆绿素还原酶活性的检测]
[Detection of biliverdin reductase activity of Schistosoma japonicum].
作者信息
Wen-qi Liu, Yong-long Li
机构信息
Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2004 Apr 30;22(2):106-8.
OBJECTIVE
To explore the presence of biliverdin reductase (BR) activity in adult worms of Schistosoma japonicum in vitro.
METHODS
The soluble fraction was isolated from homogenates of adult worms of S. japonicum, and incubated with biliverdin under different pH conditions and buffers. The time dependency of this enzymatic reaction was also detected.
RESULTS
The soluble fraction of the homogenates of adult worms could degrade biliverdin in vitro, the BR activity was 43.30 nmol/(mg x min), with its optimal condition at pH 8.7. The rate of reaction peaked at 15 min of incubation for the BR activity.
CONCLUSION
The presence of BR activity in adult worms of S. japonicum was firstly demonstrated.
目的
在体外探究日本血吸虫成虫中是否存在胆绿素还原酶（BR）活性。
方法
从日本血吸虫成虫匀浆中分离出可溶部分，并在不同pH条件和缓冲液下与胆绿素一起孵育。还检测了该酶促反应的时间依赖性。
结果
成虫匀浆的可溶部分在体外可降解胆绿素，BR活性为43.30 nmol/（mg·min），其最适条件为pH 8.7。BR活性在孵育15分钟时反应速率达到峰值。
结论
首次证实日本血吸虫成虫中存在BR活性。
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