采用固相微萃取预处理，气相色谱法测定血液中苯、甲苯、二甲苯暴露量的分析方法。
Analytical method for evaluation of exposure to benzene, toluene, xylene in blood by gas chromatography preceded by solid phase microextraction.
作者信息
Alegretti Ana Paula, Thiesen Flavia V, Maciel Gisele P
机构信息
Institute of Toxicology, Pontifícia Universidade Católica do Rio Grande do Sul, Ipiranga Av, 6681, 90619-900 Porto Alegre, RS, Brazil.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Sep 25;809(1):183-7. doi: 10.1016/j.jchromb.2004.06.016.
Frequency of intentional exposure to organic solvents has been increasing among children and adolescents in Brazil. Analysis of benzene, toluene and xylenes (BTX) in human blood is necessary to diagnose the intentional and accidental exposure to these solvents. A method for BTX determination in blood samples by gas chromatography preceded by solid phase microextration (SPME) from headspace (HS) has been described. SPME has several advantages when compared to other extraction techniques such as simplicity, low cost and solvent-free extraction. The method presents good repeatability (precision was of 2.2-8.0%), accuracy from -4.7 to -9.4%, limit of detection <1.0 ug/mL, linearity from 1.0 to 100 ug/mL for toluene and from 5.0 to 100 ug/mL for the other solvents (R2 > 0.99), which shows to be efficient and adequate for the detection of exposure to BTX in blood samples.
在巴西，儿童和青少年故意接触有机溶剂的频率一直在上升。分析人体血液中的苯、甲苯和二甲苯（BTX）对于诊断这些溶剂的故意和意外接触情况很有必要。本文描述了一种采用顶空固相微萃取（SPME）预处理后通过气相色谱法测定血液样本中BTX的方法。与其他萃取技术相比，SPME具有诸多优点，如操作简单、成本低且无需使用溶剂进行萃取。该方法具有良好的重复性（精密度为2.2 - 8.0%）、准确度在 -4.7至 -9.4%之间、检测限<1.0 μg/mL、甲苯的线性范围为1.0至100 μg/mL，其他溶剂的线性范围为5.0至100 μg/mL（R2>0.99），这表明该方法对于检测血液样本中BTX的接触情况高效且适用。
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