Chitra K C, Mathur P P
School of Life Sciences, Pondicherry University, Pondicherry 605 014, India.
Indian J Exp Biol. 2004 Feb;42(2):220-3.
In the present study we have investigated if administration of nonylphenol-induced oxidative stress in various subcellular fractions of adult rat testis and the effect of vitamin E on reactive oxygen species mediated nonylphenol toxicity. Male rats were administered orally with nonylphenol at 1, 10 and 100 microg/kg body weight per day for 45 days with and without supplementation of vitamin E (20 mg/kg body weight). In nonylphenol-treated rats the activities of antioxidant enzymes superoxide dismutase and glutathione reductase decreased significantly while the levels of lipid peroxidation increased significantly in the crude homogenate and in the mitochondrial and microsome-rich fractions of testis. Co-administration of nonylphenol and vitamin E did not cause changes in the activities of antioxidant enzymes in various subcellular fractions of rat testis. The results suggest that graded doses of nonylphenol elicit depletion of antioxidant defence system in rat testis, indicating nonylphenol induced oxidative stress in the testis of rats which could be reversed by the administration of vitamin E.
在本研究中，我们调查了壬基酚给药是否会在成年大鼠睾丸的各个亚细胞组分中诱导氧化应激，以及维生素E对活性氧介导的壬基酚毒性的影响。雄性大鼠每天按1、10和100微克/千克体重口服壬基酚，持续45天，同时补充或不补充维生素E（20毫克/千克体重）。在经壬基酚处理的大鼠中，睾丸粗匀浆、线粒体和富含微粒体的组分中抗氧化酶超氧化物歧化酶和谷胱甘肽还原酶的活性显著降低，而脂质过氧化水平显著升高。壬基酚与维生素E共同给药并未引起大鼠睾丸各个亚细胞组分中抗氧化酶活性的变化。结果表明，分级剂量的壬基酚会导致大鼠睾丸中抗氧化防御系统的耗竭，表明壬基酚在大鼠睾丸中诱导了氧化应激，而维生素E给药可逆转这种应激。
