Senthil kumar J, Banudevi S, Sharmila M, Murugesan P, Srinivasan N, Balasubramanian K, Aruldhas M M, Arunakaran J
Department of Endocrinology, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai 600113, India.
Reprod Toxicol. 2004 Dec;19(2):201-8. doi: 10.1016/j.reprotox.2004.08.001.
The effect of Aroclor 1254 and the ameliorative effect of Vitamin C and E on Sertoli cell function were studied in adult male rats. The rats were administered Aroclor 1254 at a dose of 2 mg/kg bw/day intraperitoneally for 30 days. One group of rats received Vitamin C (100 mg/kg bw/day) while the other group received Vitamin E (50 mg/kg bw/day) orally simultaneously with Aroclor 1254 for 30 days. Necropsy was performed at 24 h after the last injection. Sertoli cells were isolated for the estimation of enzymatic antioxidants superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GST), and gamma-glutamyl transpeptidase (gamma-GT). Lipid peroxidation (LPO), hydrogen peroxide and hydroxyl radical were estimated. Sertoli cellular androgen binding protein (ABP) and lactate were also quantified. Whereas body weight, testis weight, relative weight of testis, ABP, lactate and specific activities of SOD, CAT, GPx, GR, GST, gamma-GT were all decreased, the levels of hydrogen peroxide, hydroxyl radical and LPO were significantly increased in the Sertoli cells of Aroclor 1254 treated rats. Simultaneous administration of Vitamin C or E restored these parameters to a normal range. Thus, the present study suggests that Aroclor 1254 exposure induces oxidative stress in rat Sertoli cells and furthermore that simultaneous administration of Vitamin C or E ameliorated these effects.
研究了多氯联苯混合物Aroclor 1254的作用以及维生素C和E对成年雄性大鼠支持细胞功能的改善作用。大鼠腹腔注射剂量为2mg/kg体重/天的Aroclor 1254，持续30天。一组大鼠同时口服维生素C（100mg/kg体重/天），另一组大鼠同时口服维生素E（50mg/kg体重/天），并与Aroclor 1254同时给药30天。在最后一次注射后24小时进行尸检。分离支持细胞以评估酶促抗氧化剂超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽还原酶（GST）和γ-谷氨酰转肽酶（γ-GT）。评估脂质过氧化（LPO）、过氧化氢和羟自由基。还对支持细胞雄激素结合蛋白（ABP）和乳酸进行了定量。结果显示，Aroclor 1254处理的大鼠支持细胞中，体重、睾丸重量、睾丸相对重量、ABP、乳酸以及SOD、CAT、GPx、GR、GST、γ-GT的比活性均降低，而过氧化氢、羟自由基和LPO水平显著升高。同时给予维生素C或E可使这些参数恢复到正常范围。因此，本研究表明，暴露于Aroclor 1254会诱导大鼠支持细胞产生氧化应激，此外，同时给予维生素C或E可改善这些影响。
