Azizi M Sc Parisa, Soleimani Mehranjani Ph D Malek
Department of Biology, Faculty of Sciences, Arak University, Arak, Iran.
Int J Reprod Biomed. 2019 Nov 7;17(10):717-726. doi: 10.18502/ijrm.v17i10.5290. eCollection 2019 Oct.
Para-nonylphenol (p-NP), an environmental contaminant, can generate free radicals that disturbs the reproductive properties. Green tea extract (GTE) is an antioxidant which may prevent the adverse effects of free radicals. The aim was to investigate the effect of GTE on sperm parameters and testis tissue in p-NP-treated rats.
24 adult male Wistar rats (215 20 gr) were randomly divided into four groups (n = 6/each) - including control, p-NP (200 mg/kg/day), GTE (200 mg/kg/day), and p-NP + GTE - and orally treated for 56 days. The right testes and left caudal epididymis were used to evaluate selected parameters. In addition, the concentration of serum malondialdehyde was calculated.
A significant decrease in the sperm number, motility, viability and morphology (p 0.001) was observed in the rats treated with p-NP compared to the control ones. The diameter of seminiferous tubules (p 0.001), thickness of germinal epithelium (p = 0.018), total volume of testis (p = 0.009), volume of seminiferous tubules (p 0.001), and testis weight (p = 0.017) decreased in the p-NP group in contrast with the other groups. Moreover, a significant increase of the malondialdehyde concentration was seen in the p-NP group when compared with the controls (p = 0.043). The majority of adverse effects of p-NP could be recovered following the administration of GTE.
It seems GTE can be used as a potent antioxidant in the case of p-NP toxication.
对壬基酚（p-NP）作为一种环境污染物，可产生干扰生殖特性的自由基。绿茶提取物（GTE）是一种抗氧化剂，可能预防自由基的不良影响。本研究旨在探讨GTE对p-NP处理大鼠精子参数和睾丸组织的影响。
将24只成年雄性Wistar大鼠（体重215±20克）随机分为四组（每组n = 6），包括对照组、p-NP组（200毫克/千克/天）、GTE组（200毫克/千克/天）和p-NP + GTE组，并进行为期56天的口服处理。取右侧睾丸和左侧附睾尾部用于评估选定参数。此外，计算血清丙二醛浓度。
与对照组相比，p-NP处理的大鼠精子数量、活力、存活率和形态均显著下降（p < 0.001）。与其他组相比，p-NP组的生精小管直径（p < 0.001）、生精上皮厚度（p = 0.018）、睾丸总体积（p = 0.009）、生精小管体积（p < 0.001）和睾丸重量（p = 0.017）均降低。此外，与对照组相比，p-NP组的丙二醛浓度显著升高（p = 0.043）。给予GTE后，p-NP的大多数不良反应可得到恢复。
在p-NP中毒的情况下，GTE似乎可作为一种有效的抗氧化剂。
