Tatsuoka N, Mohammed N, Mitsumori M, Hara K, Kurihara M, Itabashi H
Bio Research Corporation of Yokohama, Kanagawa, Japan.
Lett Appl Microbiol. 2004;39(3):257-60. doi: 10.1111/j.1472-765X.2004.01566.x.
The object of the present study is isolation of methyl coenzyme-M reductase (MCR) genes (mcrA) from the bovine rumen fluid and determination of phylogenetical placements of the genes to investigate mechanisms of methanogenesis in the rumen from a point of view of mcrA genes.
Genes for methanogen-specific MCR were isolated from the bovine rumen by PCR amplification. The deduced amino acid sequences were fitted to the alignments of mcrA gene products from the referred sequences.
Although the deduced amino acid sequences of mcrA genes, isolated from the bovine rumen in the present study, were close to that of Methanobrevibacter ruminantium, these amino acid sequences did not fall into known clusters of MCR. The findings suggest that methanogenesis in the rumen would be partially carried out by unknown methanogens.
本研究的目的是从牛瘤胃液中分离甲基辅酶-M还原酶（MCR）基因（mcrA），并确定这些基因的系统发育位置，以便从mcrA基因的角度研究瘤胃中甲烷生成的机制。
通过PCR扩增从牛瘤胃中分离产甲烷菌特异性MCR的基因。将推导的氨基酸序列与参考序列中的mcrA基因产物比对。
尽管本研究从牛瘤胃中分离出的mcrA基因推导的氨基酸序列与反刍短杆菌的序列相近，但这些氨基酸序列并未归入已知的MCR簇。研究结果表明，瘤胃中的甲烷生成可能部分由未知的产甲烷菌进行。
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