Rastogi Gurdeep, Ranade Dilip R, Yeole Tulshiram Y, Patole Milind S, Shouche Yogesh S
Molecular Biology Unit, National Centre for Cell Science, Pune 411007, India.
Bioresour Technol. 2008 Sep;99(13):5317-26. doi: 10.1016/j.biortech.2007.11.024. Epub 2007 Dec 26.
The methanogen community in biogas reactor running on cattle dung was investigated in two different seasons; summer (April, 36 degrees C) and winter (December, 24 degrees C), in the year 2004 by a culture-independent approach. Community structure was determined by phylogenetic analyses of 343 and 278 mcrA clones belonging to summer and winter month libraries, respectively. In summer month's library, 41.7% clones were affiliated to Methanomicrobiales, 30% to Methanosarcinales, 19% to Methanobacteriales, 5% to Methanococcales and a total of 4.3% clones belonged to unclassified euryarchaeotal lineages. In winter month's library, Methanomicrobiales encompassed 98.6% clones, and Methanobacteriales included 1.4% of total clone diversity. Biogas plant performance data collected during the winter month indicated significant reduction in daily biogas produced as compared to summer month because of lowering in ambient temperature and associated shift in microbial community. Results from this molecular study showed the existence of highly diverse and complex methanogens communities present in biogas plant.
2004年，采用非培养方法对以牛粪为原料运行的沼气反应器中的产甲烷菌群落进行了两个不同季节的研究，即夏季（4月，36摄氏度）和冬季（12月，24摄氏度）。通过对分别属于夏季和冬季文库的343个和278个mcrA克隆进行系统发育分析来确定群落结构。在夏季文库中，41.7%的克隆隶属于甲烷微菌目，30%隶属于甲烷八叠球菌目，19%隶属于甲烷杆菌目，5%隶属于甲烷球菌目，共有4.3%的克隆属于未分类的广古菌谱系。在冬季文库中，甲烷微菌目包含98.6%的克隆，甲烷杆菌目占总克隆多样性的1.4%。冬季收集的沼气厂性能数据表明，由于环境温度降低和微生物群落的相关变化，与夏季相比，每日沼气产量显著降低。这项分子研究的结果表明，沼气厂中存在高度多样和复杂的产甲烷菌群落。
