Hirano A, Wang A H, Gombart A F, Wong T C
Department of Microbiology, University of Washington School of Medicine, Seattle 98195.
Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8745-9. doi: 10.1073/pnas.89.18.8745.
Persistence of measles virus in the brains of patients with subacute sclerosing panencephalitis (SSPE) is accompanied by changes in the viral matrix (M) protein. To understand the significance of these changes, cell culture and cell-free assays were developed to compare the functions of the M proteins of an SSPE virus Biken strain and its acute measles virus progenitor Nagahata strain. The Nagahata viral M protein is associated with the intracellular viral nucleocapsids and the plasma membrane, whereas the Biken viral M protein is localized mainly in the cytosol. The lack of M protein in the Biken viral nucleocapsids is due to a failure of the Biken M protein to bind to the viral nucleocapsids. The Biken M protein also fails to bind to the Nagahata viral nucleocapsids. Conversely, the Nagahata M protein can bind to the Biken viral nucleocapsids, although this association is not as stable at physiological salt concentration. These results offer concrete evidence that the M protein of an SSPE virus is functionally different from that of its progenitor acute measles virus.
亚急性硬化性全脑炎（SSPE）患者大脑中麻疹病毒的持续存在伴随着病毒基质（M）蛋白的变化。为了了解这些变化的意义，开展了细胞培养和无细胞试验，以比较SSPE病毒比肯株及其急性麻疹病毒亲本长畑株的M蛋白功能。长畑株病毒M蛋白与细胞内病毒核衣壳及质膜相关，而比肯株病毒M蛋白主要定位于胞质溶胶中。比肯株病毒核衣壳中缺乏M蛋白是由于比肯株M蛋白无法与病毒核衣壳结合。比肯株M蛋白也无法与长畑株病毒核衣壳结合。相反，长畑株M蛋白可以与比肯株病毒核衣壳结合，尽管在生理盐浓度下这种结合不太稳定。这些结果提供了确凿证据，表明SSPE病毒的M蛋白在功能上与其亲本急性麻疹病毒的M蛋白不同。
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