Morgan E M, Re G G, Kingsbury D W
Virology. 1984 May;135(1):279-87. doi: 10.1016/0042-6822(84)90137-5.
A DNA molecule representing all but the three terminal bases of the Sendai virus nucleoprotein (NP) gene, copied from viral mRNA, was inserted into pBR322. The NP insert comprised 1673 bases. The first AUG protein initiation codon, at position 65, began an open reading frame of 1551 bases, encoding a protein of 517 amino acids with an amino acid composition corresponding to previously published data. The NP gene sequence determined in the present work is similar to that described by Shioda et al. [ Nucl . Acids Res. 11, 7317 (1983)], but there are 14 amino acid differences that probably reflect differences in virus strains. The predicted secondary structure of the NP molecule and the locations within that structure of potential protease cleavage sites are in accord with structural domains previously defined by controlled protease digestion.
从病毒mRNA复制而来的、代表仙台病毒核蛋白（NP）基因除三个末端碱基外所有碱基的一个DNA分子，被插入到pBR322中。NP插入片段包含1673个碱基。位于第65位的第一个AUG蛋白质起始密码子，开启了一个1551个碱基的开放阅读框，编码一个由517个氨基酸组成的蛋白质，其氨基酸组成与先前发表的数据一致。在本研究中确定的NP基因序列与Shioda等人[《核酸研究》11, 7317 (1983)]所描述的序列相似，但存在14个氨基酸差异，这可能反映了病毒株的差异。NP分子的预测二级结构以及该结构内潜在蛋白酶切割位点的位置，与先前通过可控蛋白酶消化所定义的结构域一致。
