Domíguez-Bocanegra A R, Torres-Muñoz J A
Department of Biotechnology and Bioengineering, CINVESTAV, Avenida Instituto Politécnico Nacional 2508, Col. Zacatenco, Mexico City, DF 07300, Mexico.
Appl Microbiol Biotechnol. 2004 Dec;66(3):249-52. doi: 10.1007/s00253-004-1686-3.
Natural carbon sources, such as those present in cane sugar molasses and grape juice, promote the synthesis of astaxanthin in different Phaffia rhodozyma yeasts. One of these, coconut milk, has a very rich nutrient composition. The aim of this work was to investigate the utility of coconut milk as sole source of energy for astaxanthin pigment production by P. rhodozyma strains. Currently, coconut pulp is widely used in industrial processes in Mexico for the production of shampoos, candies, food, etc. However, coconut milk is a waste product. We show that coconut milk enhances astaxanthin production. The fermentation yielded 850 microg/g yeast with the NRRL-10921 wild-type strain and 1850 microg/g yeast with the mutated R1 strain. Production was better than reported results employing other natural carbon sources.
天然碳源,如甘蔗废蜜和葡萄汁中所含的碳源,可促进不同红法夫酵母中虾青素的合成。其中之一的椰奶,营养成分非常丰富。这项工作的目的是研究椰奶作为红法夫酵母菌株生产虾青素色素唯一能量来源的效用。目前,椰肉在墨西哥被广泛用于洗发水、糖果、食品等的工业生产过程中。然而,椰奶却是一种废品。我们发现椰奶能提高虾青素的产量。使用NRRL - 10921野生型菌株发酵,每克酵母可产生850微克虾青素;使用突变的R1菌株发酵,每克酵母可产生1850微克虾青素。其产量优于使用其他天然碳源所报道的结果。