铽敏化荧光法测定甲磺酸帕珠沙星及其应用

Terbium-sensitized fluorescence method for the determination of pazufloxacin mesilate and its application.

作者信息

Chen Shilv, Ma Hongwu, Zhao Huichun, Feng Ruiqing, Jin Linpei

机构信息

Department of Chemistry, Beijing Normal University, Beijing, PR, China.

出版信息

Anal Sci. 2004 Jul;20(7):1075-8. doi: 10.2116/analsci.20.1075.

DOI:10.2116/analsci.20.1075

PMID:15293405

Abstract

A simple, rapid and highly sensitive fluorometric method for the determination of pazufloxacin mesilate (PZFX) is described. It is based on the formation of the complex Tb(PZFX)2, which shows the intensive characteristic bands of Tb3+. Optimum conditions for the determination were investigated. Under the optimum experimental condition, the fluorescence intensity responds linearly to the PZFX concentration in the range of 2.0 x 10(-8) - 5.0 x 10(-6) mol/l with a detection limit of 6.2 x 10(-9) mol/l. The method has been successfully applied to the determination of PZFX in urine and serum samples.

摘要

描述了一种用于测定甲磺酸帕珠沙星（PZFX）的简单、快速且高度灵敏的荧光分析法。该方法基于Tb(PZFX)2配合物的形成，此配合物显示出Tb3+的强烈特征谱带。研究了测定的最佳条件。在最佳实验条件下，荧光强度与PZFX浓度在2.0×10(-8) - 5.0×10(-6) mol/L范围内呈线性响应，检测限为6.2×10(-9) mol/L。该方法已成功应用于尿液和血清样品中PZFX的测定。