[重组人骨形态发生蛋白-2促进兔下颌骨牵张成骨的骨形成]
[Recombinant human BMP-2 accelerates bone formation of mandibular distraction osteogenesis in rabbits].
作者信息
Wang Zhi-guo, Hu Jing, Zou Shu-jian, Li Ji-Hua, Gao Zhan-wei, Wang Da-zhang
机构信息
Key. Laboratory of Oral Biomedical Engineering, Ministry of Education, Sichuan University, Chengdu 610041, China.
出版信息
Hua Xi Kou Qiang Yi Xue Za Zhi. 2004 Jun;22(3):186-8.
OBJECTIVE
To study the effects of rhBMP-2 on bone formation of mandibular distraction osteogenesis in rabbits.
METHODS
Bilateral mandibular osteotomies were performed in 12 mature rabbits. 5 mg rhBMP-2 with the collagen carrier was implanted in the osteotomy site of one side of the mandibles. Only the collagen sponge was placed in the contra-lateral side as control. The mandibles of 8 rabbits were lengthened by 6mm using a custom-made distractor. At 4 weeks after the end of distraction, all animals were killed and the distracted calluses were harvested and processed for histological, scanning electron microscopic, as well as Ca/P ratio analysis.
RESULTS
The regenerated bone was found in the distraction gap after mandibular lengthening. The mandibular side treated with rhBMP-2 had greater amounts of new bone formation and earlier mineralization than contra-lateral side (non-rhBMP-2 treated).
CONCLUSION
Recombinant human BMP-2 appears to be able to accelerate bone formation of mandibular distraction osteogenesis in rabbits.
目的
研究重组人骨形态发生蛋白-2（rhBMP-2）对兔下颌骨牵张成骨中骨形成的影响。
方法
对12只成年兔进行双侧下颌骨截骨术。将5mg rhBMP-2与胶原载体植入一侧下颌骨的截骨部位。对侧仅放置胶原海绵作为对照。使用定制的牵张器将8只兔的下颌骨延长6mm。牵张结束后4周，处死所有动物，收集牵张部位的骨痂并进行组织学、扫描电子显微镜检查以及钙磷比分析。
结果
下颌骨延长后，在牵张间隙发现再生骨。与对侧（未用rhBMP-2处理）相比，用rhBMP-2处理的下颌骨侧有更多的新骨形成且矿化更早。
结论
重组人BMP-2似乎能够加速兔下颌骨牵张成骨中的骨形成。
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