Ren Ling-Fei, Shi Geng-Sheng, Tong Yong-Qing, Jiang Song-Yao, Zhang Feng
Resident, Taizhou Hospital of Zhejiang Province, Linhai, People's Republic of China.
Professor and Department Head, Taizhou Hospital of Zhejiang Province, Linhai, People's Republic of China.
J Oral Maxillofac Surg. 2018 May;76(5):1092.e1-1092.e10. doi: 10.1016/j.joms.2018.01.013. Epub 2018 Jan 31.
The effects of a recombinant human bone morphogenetic protein-2/7 (rhBMP-2/7) heterodimer and a RADA16 (Ac-RADARADARADARADA-CONH2) hydrogel scaffold on bone formation during distraction osteogenesis were evaluated.
Forty New Zealand white rabbits, which underwent mandibular lengthening, were randomly divided into 5 groups. One group served as the control group. The others received 2 μg of rhBMP-2 homodimer, 2 μg of rhBMP-2/7 heterodimer, 100 μL of RADA16, or 100 μL of RADA16 plus 2 μg of rhBMP-2/7 heterodimer in the mandibular distraction gap at the beginning of distraction. Fluorine-18-labeled fluoride positron emission tomography was used to assess osteogenesis both after distraction and at the end of consolidation. Dual-energy x-ray absorptiometry (DEXA) examination and bone histologic findings were also evaluated.
At the end of distraction, the radioactivity concentration in the distracted area was significantly greater in the RADA16 plus rhBMP-2/7 heterodimer group than in the other groups (P < .01). The differences among the other 4 groups were also statistically significant in the following order: rhBMP-2/7 heterodimer group greater than the rhBMP-2 homodimer group, which was greater than the RADA16 group (or control group; P < .05). However, the radioactivity concentration of the RADA16 group was slightly greater than that of the control group with a nonsignificant difference (P > .05). By the end of consolidation, the activity in the control group, RADA16 group, rhBMP-2 homodimer group, and rhBMP-2/7 heterodimer group had significantly diminished (P < .05). However, the activity in the RADA16 plus rhBMP-2/7 heterodimer group remained at the same level (P > .05). The DEXA results and bone histologic findings indicated that more callus regeneration was noted in the RADA16 plus rhBMP-2/7 heterodimer group than in any other group.
The use of rhBMP-2/7 heterodimer and RADA16 hydrogel scaffold significantly promoted mandibular distraction osteogenesis.
评估重组人骨形态发生蛋白-2/7（rhBMP-2/7）异二聚体和RADA16（Ac-RADARADARADARADA-CONH2）水凝胶支架对牵张成骨过程中骨形成的影响。
40只接受下颌骨延长术的新西兰白兔被随机分为5组。一组作为对照组。其他组在牵张开始时于下颌骨牵张间隙分别给予2μg rhBMP-2同二聚体、2μg rhBMP-2/7异二聚体、100μL RADA16或100μL RADA16加2μg rhBMP-2/7异二聚体。使用氟-18标记的氟化物正电子发射断层扫描来评估牵张后和巩固期结束时的成骨情况。还进行了双能X线吸收法（DEXA）检查和骨组织学检查。
牵张结束时，RADA16加rhBMP-2/7异二聚体组牵张区域的放射性浓度显著高于其他组（P <.01）。其他4组之间的差异也具有统计学意义，顺序如下：rhBMP-2/7异二聚体组大于rhBMP-2同二聚体组，rhBMP-2同二聚体组大于RADA16组（或对照组；P <.05）。然而，RADA16组的放射性浓度略高于对照组，差异无统计学意义（P >.05）。到巩固期结束时，对照组、RADA16组、rhBMP-2同二聚体组和rhBMP-2/7异二聚体组的活性显著降低（P <.05）。然而，RADA16加rhBMP-2/7异二聚体组的活性保持在同一水平（P >.05）。DEXA结果和骨组织学检查结果表明，RADA16加rhBMP-2/7异二聚体组的骨痂再生比其他任何组都更多。
rhBMP-2/7异二聚体和RADA16水凝胶支架的使用显著促进了下颌骨牵张成骨。
