Köseoğlu Ozgen, Sener Burçin, Gür Deniz
Hacettepe Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Ankara.
Mikrobiyol Bul. 2004 Jan-Apr;38(1-2):9-19.
As alternative regimens in therapy of Stenotrophomonas maltophilia infections are limited, epidemiological investigation of S. maltophilia is necessary for developing new strategies in prophylaxis of these infections. "Enterobacterial repetetive intergenic consensus-polymerase chain reaction (ERIC-PCR)", is a simple and rapid method to detect the genomic polymorphism at the strain level in nosocomial outbreaks due to S. maltophilia. The aim of this study was to investigate the molecular epidemiology of S. maltophilia strains isolated from paediatric patients in a university hospital. A total of 40 clinical isolates one from each of 35 patients, two pairs from two patients and one from an environmental source, were collected between 1998-2001 period. Susceptibility to eleven antimicrobials was studied by agar dilution test according to NCCLS criteria. Resistance rates of the tested isolates against piperacillin, piperacillin-tazobactam, ceftriaxone, ceftazidime, cefepime, ciprofloxacin, imipenem, meropenem, amikacin, gentamicin and trimethoprim-sulfamethoxazole were 57.5%, 25%, 85%, 37.5%, 47.5%, 20%, 100%, 95%, 62.5%, 62.5% and 5%, respectively. Trimethoprim-sulfamethoxazole was the most active agent against the tested isolates. The isolates were analyzed by ERIC-PCR by using each of ERIC-I and ERIC-II primers. Genotypic analysis by ERIC-PCR identified 24 different major and 4 minor profiles. Three isolates from each of four different groups, two isolates from each of other four different groups were unique. The results of this study, revealing a great genomic diversity and low clonality within the strains of S. maltophilia strains suggest that patients may be colonized with the naturally occuring isolates in the hospital environment and then get infected with these strains under the selective antibiotic pressure, thus supporting the view that clonal spread of S. maltophilia strains between patients is of rather low possibility.
由于嗜麦芽窄食单胞菌感染的替代治疗方案有限，因此有必要对嗜麦芽窄食单胞菌进行流行病学调查，以制定预防这些感染的新策略。“肠杆菌基因间重复一致序列聚合酶链反应（ERIC-PCR）”是一种简单快速的方法，可用于检测嗜麦芽窄食单胞菌所致医院感染暴发中菌株水平的基因组多态性。本研究的目的是调查从一所大学医院儿科患者中分离出的嗜麦芽窄食单胞菌菌株的分子流行病学。在1998年至2001年期间，共收集了40株临床分离株，其中35名患者每人一株，两名患者各有两对，还有一株来自环境源。根据NCCLS标准，采用琼脂稀释法研究了这些菌株对11种抗菌药物的敏感性。受试菌株对哌拉西林、哌拉西林-他唑巴坦、头孢曲松、头孢他啶、头孢吡肟、环丙沙星、亚胺培南、美罗培南、阿米卡星、庆大霉素和复方磺胺甲恶唑的耐药率分别为57.5%、25%、85%、37.5%、47.5%、20%、100%、95%、62.5%、62.5%和5%。复方磺胺甲恶唑是对受试菌株最有效的药物。使用ERIC-I和ERIC-II引物分别对分离株进行ERIC-PCR分析。ERIC-PCR的基因分型鉴定出24种不同的主要图谱和4种次要图谱。来自四个不同组的每组中的三株分离株，以及其他四个不同组的每组中的两株分离株是独特的。本研究结果显示嗜麦芽窄食单胞菌菌株内存在很大的基因组多样性和低克隆性，这表明患者可能在医院环境中被自然存在的分离株定植，然后在选择性抗生素压力下感染这些菌株，从而支持了嗜麦芽窄食单胞菌菌株在患者之间克隆传播可能性相当低的观点。
Zotero 插件