慢性活动性EB病毒感染患者中EB病毒特异性CD8 + T细胞的定量分析。

Quantitative analysis of Epstein-Barr virus (EBV)-specific CD8+ T cells in patients with chronic active EBV infection.

作者信息

Sugaya Naomi, Kimura Hiroshi, Hara Shinya, Hoshino Yo, Kojima Seiji, Morishima Tsuneo, Tsurumi Tatsuya, Kuzushima Kiyotaka

机构信息

Department of Pediatrics, Nagoya University Graduate School of Medicine, Tsurumai-cho, Showa-ku, Japan.

出版信息

J Infect Dis. 2004 Sep 1;190(5):985-8. doi: 10.1086/423285. Epub 2004 Jul 23.

DOI:10.1086/423285

PMID:15295706

Abstract

To clarify the pathogenesis of chronic active Epstein-Barr virus (EBV) infection, EBV-specific CD8+ T cells were enumerated, by use of human leukocyte antigen (HLA)-A*2402-restricted tetramers, in 8 patients with chronic active EBV infection, 10 patients with infectious mononucleosis, and 16 EBV-seropositive healthy control subjects. In most of the patients with chronic active EBV infection, EBV-specific CD8+ T cells were not detected. Of note, latent membrane protein 2-specific CD8+ T cells were not detectable in any patients with chronic active EBV infection. In contrast, EBV-specific CD8+ T cells were detected in patients with infectious mononucleosis and in healthy control subjects. Low frequencies of EBV-specific CD8+ T cells may be one of the immunological features of chronic active EBV infection.

摘要

为阐明慢性活动性EB病毒（EBV）感染的发病机制，我们使用人类白细胞抗原（HLA）-A*2402限制性四聚体，对8例慢性活动性EBV感染患者、10例传染性单核细胞增多症患者和16例EBV血清学阳性健康对照者的EBV特异性CD8+T细胞进行了计数。在大多数慢性活动性EBV感染患者中，未检测到EBV特异性CD8+T细胞。值得注意的是，在任何慢性活动性EBV感染患者中均未检测到潜伏膜蛋白2特异性CD8+T细胞。相比之下，在传染性单核细胞增多症患者和健康对照者中检测到了EBV特异性CD8+T细胞。EBV特异性CD8+T细胞的低频率可能是慢性活动性EBV感染的免疫特征之一。