梭子鱼4.10β-小清蛋白新晶型的X射线结构

X-ray structure of a new crystal form of pike 4.10 beta parvalbumin.

作者信息

Declercq J P, Tinant B, Parello J

机构信息

Laboratoire de chimie physique et de cristallographie, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.

出版信息

Acta Crystallogr D Biol Crystallogr. 1996 Jan 1;52(Pt 1):165-9. doi: 10.1107/S0907444995010006.

DOI:10.1107/S0907444995010006

PMID:15299738

Abstract

A new crystal form of pike (pI 4.10) parvalbumin has been crystallized in presence of EDTA at pH 8.0. The crystals are orthorhombic, space group P2(1)2(1)2(1), with a = 51.84, b = 49.95, c = 34.96 A. Diffractometer data were collected to 1.75 A. The structure was solved by molecular replacement and refined to R = 0.168 for 7774 observed reflections [I>/= 2sigma(I)] in the range 8.0-1.75 A. In spite of the presence of EDTA, calcium ions are present in both primary binding sites. As compared to the previously reported structures, the main differences concern the conformation of the N-terminal residues and the packing in the unit cell.

摘要

一种新晶型的梭子鱼（pI 4.10）小清蛋白在pH 8.0的乙二胺四乙酸（EDTA）存在下结晶。晶体为正交晶系，空间群P2(1)2(1)2(1)，a = 51.84，b = 49.95，c = 34.96 Å。使用衍射仪收集数据至1.75 Å。通过分子置换法解析结构，并对8.0 - 1.75 Å范围内7774个观测反射[I>/= 2σ(I)]进行精修，最终R值为0.168。尽管存在EDTA，但在两个主要结合位点均存在钙离子。与先前报道的结构相比，主要差异在于N端残基的构象以及晶胞中的堆积情况。