Kravchenko A N, Mishan'kin B N
Antibiot Khimioter. 1992 Jan;37(1):17-20.
The comparative study revealed thymidine phosphorylase activity in the initial strains of a plague microbe of the field variety and in thymidine-, thymine-dependent and trimethoprim-resistant mutants of the plague microbe of other varieties. The data fully conformed to the results of the microbiological investigation of the strains' ability to grow on the nutrient media with trimethoprim in the presence of thymine and thymidine. On the basis of these results it appeared possible to divide the initial and mutant strains of the plague microbe into four arbitrary groups: initial strains of the plague microbe of all the varieties except the field ones sensitive to trimethoprim under any temperature conditions of incubation on any medium with any supplements; initial strains of the plague microbe of the field variety resistant to trimethoprim at 28 degrees C in the presence of thymine or thymidine alone; Tmpr mutants whose resistance to trimethoprim at 28 degrees C did not depend on the presence of thymine or thymidine, purine and vitamins, but depended on the presence of these substances at a temperature of 37 degrees C.
比较研究揭示了野毒株鼠疫杆菌初始菌株以及其他变种的鼠疫杆菌的胸苷磷酸化酶活性，这些变种包括对胸苷、胸腺嘧啶有依赖性的以及对甲氧苄啶有抗性的突变株。这些数据与菌株在含有甲氧苄啶的营养培养基上，在胸腺嘧啶和胸苷存在的情况下生长能力的微生物学研究结果完全相符。基于这些结果，似乎有可能将鼠疫杆菌的初始菌株和突变菌株任意分为四组：除野毒株外，所有变种的鼠疫杆菌初始菌株在任何温度条件下，于添加任何物质的任何培养基上对甲氧苄啶敏感；野毒株鼠疫杆菌初始菌株在28℃时，仅在胸腺嘧啶或胸苷存在的情况下对甲氧苄啶有抗性；Tmpr突变株在28℃时对甲氧苄啶的抗性不依赖于胸腺嘧啶或胸苷、嘌呤和维生素的存在，但在37℃时依赖于这些物质的存在。
