从对紫外线敏感的枯草芽孢杆菌菌株中分离并鉴定无胸腺嘧啶突变体。
Isolation and characterization of thymineless mutants from ultraviolet-sensitive Bacillus subtilis strains.
作者信息
Felkner I C
出版信息
J Bacteriol. 1970 Nov;104(2):1030-2. doi: 10.1128/jb.104.2.1030-1032.1970.
Abstract
Thymineless mutants of Bacillus subtilis 168 ind, hcr-9 were isolated by using trimethoprim. These and other Thy(-) strains differed drastically from Thy(+) ones in their patterns of [(3)H]thymidine uptake and growth in trimethoprim-containing medium. Transformation was negligible between most mutants derived from the ultraviolet-sensitive strain 168 ind, hcr-9 but significant between 168 ind, thy and these mutants. The latter and these new mutants all grow in the presence of trimethoprim plus thymidine or thymine and fail to grow if thymine or thymidine is omitted.
摘要
使用甲氧苄啶分离出枯草芽孢杆菌168 ind、hcr - 9的无胸腺嘧啶突变体。这些以及其他胸腺嘧啶缺陷型（Thy(-)）菌株在含甲氧苄啶培养基中的[³H]胸腺嘧啶核苷摄取模式和生长情况与胸腺嘧啶正常型（Thy(+)）菌株有很大差异。源自紫外线敏感菌株168 ind、hcr - 9的大多数突变体之间的转化可忽略不计，但168 ind、thy与这些突变体之间的转化则很显著。后者以及这些新突变体在甲氧苄啶加胸腺嘧啶核苷或胸腺嘧啶存在的情况下均能生长，但若省略胸腺嘧啶或胸腺嘧啶核苷则无法生长。
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