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家猫中主要卫星DNA家族(FA-SAT)的染色体定位

Chromosomal localization of the major satellite DNA family (FA-SAT) in the domestic cat.

作者信息

Santos S, Chaves R, Guedes-Pinto H

机构信息

Department of Genetics and Biotechnology, Centre of Genetics and Biotechnology of the University of Trás-os-Montes and Alto Douro-CGB/UTAD, Vila Real, Portugal.

出版信息

Cytogenet Genome Res. 2004;107(1-2):119-22. doi: 10.1159/000079581.

Abstract

A major satellite DNA sequence was isolated from the cat genome and its sequencing data revealed homology to the FA-SAT family. In situ hybridization of the cat satellite DNA and telomeric sequences to cat chromosomes, together with staining of constitutive heterochromatin, allowed the physical mapping of the FA-SAT sequences, and also an overall constitutive heterochromatin study in cat chromosomes.

摘要

从猫基因组中分离出一个主要的卫星DNA序列,其测序数据显示与FA-SAT家族具有同源性。将猫卫星DNA和端粒序列与猫染色体进行原位杂交,同时对组成型异染色质进行染色,从而实现了FA-SAT序列的物理定位,以及对猫染色体整体组成型异染色质的研究。

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