Dipartimento di Genetica e Microbiologia Adriano Buzzati-Traverso, Università di Pavia, Pavia, Italy.
Hereditas. 2011 Jun;148(3):110-3. doi: 10.1111/j.1601-5223.2011.02218.x. Epub 2011 May 12.
In the karyotype of Equus asinus (domestic donkey, 2n = 62), non-centromeric heterochromatic bands have been described in subcentromeric and telomeric positions. In particular, chromosome 1 is characterised by heterochromatic bands in the proximal region of the long arm and in the short arm; it has been shown that these regions are polymorphic in size. Here we investigated the variation in the intensity and distribution of fluorescence signals observed on donkey chromosome 1 after in situ hybridization with two DNA probes containing fragments from the two major equine satellite DNA families. Our results show that, in Equus asinus chromosome 1, the amount and distribution of large clusters of satellite DNA can define at least nine polymorphic variants of the constitutive heterochromatin that cannot be detected by C-banding alone.
在马科动物的核型中（家驴，2n=62），已经在亚着丝粒和端粒位置描述了非着丝粒异染色质带。特别是，染色体 1 具有长臂近端和短臂异染色质带的特征；已经表明，这些区域在大小上是多态的。在这里，我们使用两个包含来自两个主要马卫星 DNA 家族的片段的 DNA 探针，研究了在原位杂交后观察到的驴染色体 1 上荧光信号的强度和分布的变化。我们的结果表明，在驴染色体 1 中，卫星 DNA 的大量簇的数量和分布可以定义至少九个不能仅通过 C 带化检测到的组成型异染色质的多态变体。
