Tomiya T, Nagoshi S, Fujiwara K
First Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan.
Hepatology. 1992 Jan;15(1):1-4. doi: 10.1002/hep.1840150102.
Serum human hepatocyte growth factor levels were measured using a newly developed enzyme-linked immunosorbent assay kit in patients with liver diseases. Serum human hepatocyte growth factor levels were increased in correlation with derangements of prothrombin time, total bilirubin and other parameters reflecting hepatocellular dysfunction in 112 patients with chronic liver disease. The levels were positively correlated with serum AST and ALT levels in 59 of these patients whose prothrombin times were within the normal range. Abnormally increased serum human hepatocyte growth factor levels were found in 100% of the determinations in 16 patients with fulminant hepatic failure and in 80% of the determinations in 16 patients with chronic hepatic failure. The levels greater than 1 ng/ml, however, were found in 94% of determinations in the former group, but only in 16% of the determinations in the latter group. This difference was seen irrespective of prothrombin time or hepatic coma grades. In patients with fulminant hepatic failure serum human hepatocyte growth factor levels were increased immediately after plasma exchange using heparin as the anticoagulant in 71% of the determinations. This increase disappeared 12 hr after discontinuation of plasma exchange. In 17 of 39 patients with chronic renal failure who had no liver disease, serum human hepatocyte growth factor levels were abnormally increased before hemodialysis using heparin, and the levels were elevated immediately after hemodialysis in all the patients. The increase of serum human hepatocyte growth factor levels in hepatic failure may be the result of hepatocellular dysfunction and necrosis.(ABSTRACT TRUNCATED AT 250 WORDS)
采用新开发的酶联免疫吸附测定试剂盒，检测肝病患者血清中人肝细胞生长因子水平。112例慢性肝病患者血清中人肝细胞生长因子水平与凝血酶原时间、总胆红素及反映肝细胞功能障碍的其他参数紊乱相关升高。在其中59例凝血酶原时间在正常范围内的患者中，该水平与血清谷草转氨酶和谷丙转氨酶水平呈正相关。在16例暴发性肝衰竭患者的所有检测中，以及16例慢性肝衰竭患者80%的检测中，均发现血清中人肝细胞生长因子水平异常升高。然而，在前一组94%的检测中发现该水平大于1 ng/ml，而在后一组中仅16%的检测中发现该水平大于1 ng/ml。无论凝血酶原时间或肝昏迷分级如何，均可见这种差异。在暴发性肝衰竭患者中，71%的检测结果显示，以肝素作为抗凝剂进行血浆置换后，血清中人肝细胞生长因子水平立即升高。血浆置换停止12小时后，这种升高消失。在39例无肝病的慢性肾衰竭患者中，17例在使用肝素进行血液透析前血清中人肝细胞生长因子水平异常升高，且所有患者在血液透析后该水平立即升高。肝衰竭时血清中人肝细胞生长因子水平升高可能是肝细胞功能障碍和坏死的结果。（摘要截选至250字）
