Murgia Irene, de Pinto Maria Concetta, Delledonne Massimo, Soave Carlo, De Gara Laura
Sezione di Fisiologia e Biochimica delle Piante, Dipartimento di Biologia, Università degli Studi di Milano, via Celoria, Milano, Italy.
J Plant Physiol. 2004 Jul;161(7):777-83. doi: 10.1016/j.jplph.2003.12.004.
Past studies investigating the regulatory functions of nitric oxide (NO) in plant cells have utilized various NO-donors that release NO in different redox forms, which has lead to problems in the interpretation of data. In the present study, the effects of different NO-donors releasing NO with either NO+ (SNP) or NO' (SNAP, GSNO, NOC-18) character have been compared in plant cells. In particular, ferritin regulation, programmed cell death, cellular redox state, and ROS-scavenging enzymes in Arabidopsis thaliana and Nicotiana tabacum cells were examined. The results show that SNP behaves differently than the other NO-donors tested; indeed, SNP induces accumulation of ferritin transcripts in Arabidopsis, whereas SNAP inhibits its accumulation. Moreover, among the assortment of donors tested, only SNP caused programmed cell death and suppression of ROS-scavenging systems.
过去研究一氧化氮（NO）在植物细胞中的调节功能时，使用了各种以不同氧化还原形式释放NO的NO供体，这导致了数据解释方面的问题。在本研究中，比较了具有NO⁺（SNP）或NO⁻（SNAP、GSNO、NOC-18）特性的不同NO供体在植物细胞中释放NO的效果。特别地，研究了拟南芥和烟草细胞中的铁蛋白调节、程序性细胞死亡、细胞氧化还原状态和ROS清除酶。结果表明，SNP的行为与其他测试的NO供体不同；事实上，SNP诱导拟南芥中铁蛋白转录本的积累，而SNAP则抑制其积累。此外，在所测试的供体种类中，只有SNP导致程序性细胞死亡并抑制ROS清除系统。
