Woźniak Agnieszka, Formela Magda, Bilman Piotr, Grześkiewicz Katarzyna, Bednarski Waldemar, Marczak Łukasz, Narożna Dorota, Dancewicz Katarzyna, Mai Van Chung, Borowiak-Sobkowiak Beata, Floryszak-Wieczorek Jolanta, Gabryś Beata, Morkunas Iwona
Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland.
Institute of Molecular Physics, Polish Academy of Sciences, Smoluchowskiego 17, 60-179 Poznań, Poland.
Int J Mol Sci. 2017 Feb 5;18(2):329. doi: 10.3390/ijms18020329.
The aim of this study was to investigate the effect of exogenous nitric oxide (NO), i.e., -nitrosoglutathione (GSNO) and sodium nitroprusside (SNP), on the metabolic status of L. cv. Cysterski leaves infested by Harris, population demographic parameters and feeding activity. A reduction in the level of semiquinone radicals in pea seedling leaves pretreated with exogenous NO occurred 24 h after infestation, which was earlier than in non-pretreated leaves. A decrease in the level of O₂ was observed in leaves pretreated with GSNO and infested by aphids at 48 and 72 h post-infestation (hpi). Directly after the pretreatment with GSNO, an increase in the level of metal ions was recorded. NO considerably induced the relative mRNA levels for phenylalanine ammonia-lyase in 24-h leaves pretreated with NO donors, both non-infested and infested. NO stimulated the accumulation of pisatin in leaves until 24 h. The Electrical Penetration Graph revealed a reduction in the feeding activity of the pea aphid on leaves pretreated with NO. The present study showed that foliar application of NO donors induced sequentially defense reactions of pea against and had a deterrent effect on aphid feeding and limited the population growth rate.
本研究的目的是探究外源性一氧化氮（NO），即亚硝基谷胱甘肽（GSNO）和硝普钠（SNP）对受哈里斯蚜虫侵害的豌豆（L. cv. Cysterski）叶片代谢状态、种群统计学参数及取食活性的影响。用外源性NO预处理的豌豆幼苗叶片在受侵害24小时后，半醌自由基水平降低，这比未预处理的叶片更早出现。在受蚜虫侵害后48小时和72小时，用GSNO预处理且受蚜虫侵害的叶片中观察到O₂水平下降。在用GSNO预处理后立即记录到金属离子水平升高。NO显著诱导了用NO供体预处理24小时的叶片中苯丙氨酸解氨酶的相对mRNA水平，无论叶片是否受侵害。NO刺激叶片中豌豆素的积累直至24小时。刺吸电位图谱显示，用NO预处理的叶片上豌豆蚜的取食活性降低。本研究表明，叶面喷施NO供体可依次诱导豌豆对蚜虫的防御反应，对蚜虫取食具有威慑作用，并限制种群增长率。
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