Imai Ryozo, Ali Asghar, Pramanik Habibur R, Nakaminami Kentaro, Sentoku Naoki, Kato Hideki
Winter Stress Laboratory, Department of Low Temperature Sciences, National Agricultural Research Center for Hokkaido Region, Hitsujigaoka, Toyohira-ku, Sapporo, Japan.
J Plant Physiol. 2004 Jul;161(7):883-6. doi: 10.1016/j.jplph.2003.11.002.
A cDNA for a putative 42 kD spermidine synthase (OsSPDS2) was cloned from rice. The deduced OsSPDS2 sequence showed highest similarity with Arabidopsis AtSPDS3. Phylogenetic analysis revealed that OsSPDS2 and AtSPDS3 form a distinctive subclass in the spermidine synthase family in plants. OsSPDS2 mRNA accumulated in roots during long term exposure to chilling temperature (12 degrees C). In contrast, no such induction of the paralogous OsSPDS1 was observed during the chilling treatment. ABA treatment up-regulated OsSPDS2, whereas salt stress did not change OsSPDS2 levels significantly. Data suggested a distinct function of OsSPDS2 in chilling response in rice.
从水稻中克隆出一个假定的42kD亚精胺合酶（OsSPDS2）的cDNA。推导的OsSPDS2序列与拟南芥AtSPDS3的相似性最高。系统发育分析表明，OsSPDS2和AtSPDS3在植物亚精胺合酶家族中形成一个独特的亚类。长期暴露在低温（12摄氏度）下时，OsSPDS2 mRNA在根中积累。相反，在低温处理期间未观察到同源的OsSPDS1有这种诱导现象。ABA处理上调了OsSPDS2，而盐胁迫并未显著改变OsSPDS2的水平。数据表明OsSPDS2在水稻的低温响应中具有独特功能。
