通过pH敏感脂质体递送至树突状细胞的可溶性蛋白在体外诱导原发性细胞毒性T淋巴细胞反应。
Soluble proteins delivered to dendritic cells via pH-sensitive liposomes induce primary cytotoxic T lymphocyte responses in vitro.
作者信息
Nair S, Zhou F, Reddy R, Huang L, Rouse B T
机构信息
Department of Microbiology, College of Veterinary Medicine, University of Tennessee, Knoxville 37996.
出版信息
J Exp Med. 1992 Feb 1;175(2):609-12. doi: 10.1084/jem.175.2.609.
Abstract
Effective immunity to many infectious agents, particularly viruses, requires a CD8+ cytotoxic T lymphocyte (CTL) response. Understanding how to achieve CTL induction with soluble proteins is important for vaccine development since such antigens are usually not processed appropriately to induce CTL. In the present report, we have demonstrated that a potent primary CTL response against a soluble protein can be achieved by delivering antigen in pH-sensitive liposomes to dendritic cells (DC) either in vivo or in vitro. Since the pH-sensitive liposome delivery system is efficient and easy to use, the approach promises to be valuable both in the study of basic mechanisms in antigen processing, and as a practical means of immunization.
摘要
对许多传染原，尤其是病毒产生有效的免疫，需要CD8 + 细胞毒性T淋巴细胞（CTL）反应。了解如何用可溶性蛋白诱导CTL对于疫苗开发很重要，因为这类抗原通常不能被适当地加工以诱导CTL。在本报告中，我们已经证明，通过在体内或体外将抗原递送至树突状细胞（DC）的pH敏感脂质体中，可以实现针对可溶性蛋白的强大的初始CTL反应。由于pH敏感脂质体递送系统高效且易于使用，该方法有望在抗原加工的基本机制研究中具有价值，并且作为一种实际的免疫手段。
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