Pohl Ehmke, Ristau Uwe, Gehrmann Thomas, Jahn Doris, Robrahn Bernd, Malthan Dirk, Dobler Hannes, Hermes Christoph
European Molecular Biology Laboratory Hamburg Outstation, Notkestrasse 85, D-22603 Hamburg, Germany.
J Synchrotron Radiat. 2004 Sep 1;11(Pt 5):372-7. doi: 10.1107/S090904950401516X. Epub 2004 Aug 17.
The EMBL Hamburg Outstation currently operates five synchrotron beamlines for protein crystallography. The strongest of these beamlines is the fixed-energy beamline BW7B which receives about half of the radiation (1.5 mrad) from a 56 pole wiggler located at the DORIS III storage ring at the German synchrotron facility DESY. Over the last years this beamline has been upgraded and equipped with a fully automated crystallographic end-station and a robotic sample changer. The current set-up allows for remote operation, controlled from the user's area, of sample mounting, centering and data collection of pre-frozen crystals mounted in Hampton-type cryovials on magnetic caps. New software and intuitive graphical user interfaces have been developed that control the complete beamline set-up. Furthermore, algorithms for automatic sample centering based on UV fluorescence are being developed and combined with strategy programs in order to further automate the collection of entire diffraction data sets.
欧洲分子生物学实验室汉堡分站目前运行着五条用于蛋白质晶体学研究的同步加速器光束线。其中最强的光束线是固定能量光束线BW7B，它接收来自德国同步加速器设施DESY的DORIS III储存环上一个56极波荡器约一半的辐射（1.5毫弧度）。在过去几年里，这条光束线进行了升级，配备了全自动晶体学终端站和机器人样品更换器。目前的设置允许在用户区域远程操作，对安装在磁性帽上的汉普顿型冷冻管中的预冻晶体进行样品安装、定心和数据收集。已经开发了新的软件和直观的图形用户界面来控制整个光束线设置。此外，基于紫外荧光的自动样品定心算法正在开发，并与策略程序相结合，以进一步实现整个衍射数据集收集的自动化。
