鸡胚肝脏中不同胶原葡萄糖基转移酶反应机制的研究（高尔基体、滑面内质网、粗面内质网）

Studies on the mechanism of different collagen glucosyltransferase reactions (Golgi apparatus, smooth endoplasmic reticulum, rough endoplasmic reticulum) in chick embryo liver.

Bortolato M, Radisson J, Azzar G, Got R

Laboratoire de Biochimie des Membranes (LBTM CNRS UMR 24) Université Claude Bernard Lyon I, Villeurbanne, France.

Int J Biochem. 1992 Feb;24(2):243-8. doi: 10.1016/0020-711x(92)90253-w.

Abstract

The galactosylhydroxylysylglucosyltransferase (GGT) specific to collagen is located in the RER (rough endoplasmic reticulum), SER (smooth endoplasmic reticulum) and Golgi apparatus for the chick embryo liver. 2. The UDP-glucose collagen glucosyltransferase activities in chick embryo liver were solubilized by Nonidet P-40. 3. The mechanism of collagen glucosyltransferase reaction was studied with enzyme preparation of Golgi apparatus CF2, smooth endoplasmic reticulum CF4 and rough endoplasmic reticulum CF8. 4. For the three fractions, data obtained in experiments were consistent with a sequential ordered mechanism in which the substrates are bound to the enzyme in the following order: Mn2+, collagen and UDP-glucose substrate, with different values for Km and Vmax.

摘要

胶原蛋白特异性的半乳糖基羟赖氨酰葡糖基转移酶（GGT）位于鸡胚肝脏的粗面内质网（RER）、滑面内质网（SER）和高尔基体中。2. 用诺乃洗涤剂P-40可使鸡胚肝脏中的UDP-葡萄糖胶原蛋白葡糖基转移酶活性溶解。3. 用高尔基体CF2、滑面内质网CF4和粗面内质网CF8的酶制剂研究了胶原蛋白葡糖基转移酶反应的机制。4. 对于这三个组分，实验获得的数据与一种顺序有序机制一致，在该机制中，底物按以下顺序与酶结合：Mn2+、胶原蛋白和UDP-葡萄糖底物，Km和Vmax值不同。