鸡胚肝脏中UDP-葡萄糖胶原葡萄糖基转移酶活性的溶解：高尔基体、光滑内质网和粗糙内质网。
Solubilization of UDP-glucose collagen glucosyltransferase activities from chick embryo liver: Golgi apparatus, smooth and rough endoplasmic reticulum.
作者信息
Bortolato M, Azzar G, Farjanel J, Got R
机构信息
Laboratoire de Biochemie des Membranes (LBTM CNRS UM 380024), Université Claude Bernard Lyon I, Villeurbanne, France.
出版信息
Int J Biochem. 1991;23(9):897-900. doi: 10.1016/0020-711x(91)90077-z.
PMID:1837779
Abstract
- The choice of a suitable detergent for solubilization of UDP-glucose collagen glucosyltransferase (GGT) activities from chick embryo liver has been investigated. Several detergents were used (zwitterionic detergent as Chaps, and non-ionic detergents as Triton X-100, Nonidet P 40, Brij 35). 2. All the detergents with GGT activities were tested in Golgi apparatus, smooth and rough endoplasmic reticulum (SER, RER). 3. 80-100% GGT Golgi apparatus activity was easily solubilized at low concentrations in surfactant (0.5 mg/ml). 25-78% of SER and RER GGT activities were extracted at this concentration. 4. A higher level of detergent (5 mg/ml) was necessary to release all GGT activities of SER and RER. Protein extraction was identical to GGT activities.
摘要
- 已对用于从鸡胚肝脏中增溶UDP-葡萄糖胶原葡萄糖基转移酶（GGT）活性的合适去污剂的选择进行了研究。使用了几种去污剂（两性离子去污剂如Chaps，非离子去污剂如Triton X-100、Nonidet P 40、Brij 35）。2. 对所有具有GGT活性的去污剂在高尔基体、滑面和粗面内质网（SER、RER）中进行了测试。3. 80 - 100%的高尔基体GGT活性在表面活性剂低浓度（0.5 mg/ml）下很容易增溶。在此浓度下，25 - 78%的SER和RER GGT活性被提取出来。4. 需要更高水平的去污剂（5 mg/ml）来释放SER和RER的所有GGT活性。蛋白质提取与GGT活性相同。
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