Liu Zhi-ping, Chen Xin, She Yi-wen
Department of Forensic Medicine, Medical Center of Fudan University, Shanghai 200032, China.
Fa Yi Xue Za Zhi. 2004;20(2):68-9.
To investigate the relationship between postmortem interval (PMI) and the metabolic law of the amount of DNA in cells.
After different PMI from the heart, liver, spleen and kidney were taken into pieces respectively, then centrifuged and digested to get suspending cells fluid. The amount of DNA of rats'viscera were detected by flow cytometry after stained by fluorescence, and also inspect the amount of DNA in different periods to find out the law of its variation.
It showed a descendent trend of the amount of DNA in cells after different PMI, especially in spleen.
The amount of DNA of all the viscera grows downwards after death, this might be applyed in forensic estimation of PMI.
探讨死后间隔时间（PMI）与细胞内DNA含量代谢规律之间的关系。
取不同PMI后的心脏、肝脏、脾脏和肾脏分别进行切碎，然后离心并消化以获得悬浮细胞液。用荧光染色后通过流式细胞术检测大鼠内脏的DNA含量，并在不同时间段检测DNA含量以找出其变化规律。
不同PMI后细胞内DNA含量呈下降趋势，尤其是在脾脏中。
死后所有内脏的DNA含量均下降，这可能应用于PMI的法医学估计。
