Lin Xu, Yin Ya-sha, Ji Qiang
Department of Forensic Medicine, School of Basic Medical Science, Nanjing Medical University, Nanjing 210029, China.
Fa Yi Xue Za Zhi. 2011 Feb;27(1):47-9, 53.
Estimation of postmortem interval (PMI) is one of the difficult problems in forensic medicine. With the development of molecular biological techniques, DNA quantification methods were widely applied in estimating PMI. The postmortem degradation of DNA in different tissues and organs was discussed in this article and the recent DNA quantitative techniques being used for estimating PMI were reviewed. These techniques included single cell gel electrophoresis, Feulgen staining image analysis, flow cytometry.
死后间隔时间（PMI）的估计是法医学中的难题之一。随着分子生物学技术的发展，DNA定量方法被广泛应用于PMI的估计。本文讨论了不同组织和器官中DNA的死后降解情况，并综述了目前用于估计PMI的DNA定量技术。这些技术包括单细胞凝胶电泳、福尔根染色图像分析、流式细胞术。
