Rodríguez-Acosta A, Peña L, Finol H J, Pulido-Méndez M
Section of Immunochemistry, Tropical Medicine Institute, Central University of Venezuela, Caracas, Venezuela.
J Submicrosc Cytol Pathol. 2004 Jan;36(1):91-6.
Biopsy specimens of cervico-scutular muscles obtained from animals injected with bee crude venom were prepared for electron microscopy studies. At 6 h from Apis mellifera venom injection, in mice under transmission electron microscopy, the muscular fibres presented different atrophy levels with increment of the intermyofibrillar spaces. Tubules and sarcoplasmic reticulum elements were altered, in some places only tubular fragments and an increment of the intermyofibrillar spaces were noticed as well as loss of fibre regularity and prominent triads. In subsarcolemma region, areas lacking myofibrils and mitochondria damages were observed. Muscular segmental necrosis and atrophy areas were observed. Neuromuscular junctions were altered. The number of synaptic vesicles was very variable and synaptic clefts showed irregularities. A decrease in the number and arrangement of the synaptic clefts, as well as free polysomes, suggesting regeneration processes, were also observed. The myelinic nerves exhibited in the axon or in the wall vacuolisation areas. The presence of severe muscular lesions, the finding of venom activities in the presynaptic region and the detection of damages in the neuromuscular junctions at different chronological stages of our experiments indicate that the bee venom is highly toxic for neuromuscular structures.
对注射蜜蜂粗毒液的动物获取的颈盾肌活检标本进行电子显微镜研究准备。在注射意大利蜜蜂毒液6小时后，在透射电子显微镜下观察小鼠，肌纤维呈现不同程度的萎缩，肌原纤维间间隙增大。小管和肌浆网元件发生改变，在某些部位仅观察到管状碎片、肌原纤维间间隙增大，以及纤维规则性丧失和明显的三联体。在肌膜下区域，观察到缺乏肌原纤维的区域和线粒体损伤。观察到肌肉节段性坏死和萎缩区域。神经肌肉接头发生改变。突触小泡数量变化很大，突触间隙不规则。还观察到突触间隙数量和排列减少，以及游离多聚核糖体，提示有再生过程。髓鞘神经在轴突或壁部出现空泡化区域。在我们实验的不同时间阶段，出现严重的肌肉损伤、突触前区域毒液活性的发现以及神经肌肉接头损伤的检测，表明蜜蜂毒液对神经肌肉结构具有高度毒性。
