Rodríguez-Acosta A, Vega J, Finol H J, Pulido-Mendez M
Section of Immunochemistry of the Tropical Medicine Institute, Central University of Venezuela, Caracas, Venezuela.
J Submicrosc Cytol Pathol. 2003 Jul;35(3):309-14.
Bee accidents incidence is underestimated because many people do not consult to the physicians. Here it is described for the first time the severe mice adrenal gland damage induced by Apis mellifera venom. Biopsy specimens were obtained from mice adrenal gland and after sample preparation observed in Hitachi H-7100 electron microscope. In this work the ultrastructural analysis showed, 6 h after injection, a non homogeneous smooth endothelial reticulum, and in some places loss of plasma membrane. The fenestrae spaces were bigger and detritus in the capillary lumen were observed. Erythrocytes were seen in a cortical cell. After 48 h of venom injection, expanded fenestrae were observed. Capillary basal membrane was interrupted. Myelin-like figures and autophagic vacuoles were noticed. Swollen smooth endoplasmic reticulum elements and endothelial unfolding to the light were seen. Moreover, swollen Golgi and mitochondria were observed, in some places forming myelinic-like figures. At 144 h after venom injection, widened spaces were noticed in capillary fenestrae. Cellular section showed swollen and lost smooth endoplasmic reticulum elements. Smooth endoplasmic reticulum tubules disappearance suggested non steroidogenesis. In conclusion, we suggest that some of the bee envenoming human clinical manifestations, as is observed in mice, are determined by suprarenal gland damage produced by toxins present in this venom.
蜜蜂蜇伤事故的发生率被低估了，因为许多人不会去看医生。本文首次描述了意大利蜜蜂毒液对小鼠肾上腺造成的严重损伤。从小鼠肾上腺获取活检标本，经过样本制备后在日立H - 7100电子显微镜下观察。在这项研究中，超微结构分析显示，注射毒液6小时后，内皮网状结构不均匀且光滑，部分区域细胞膜缺失。窗孔间隙增大，可见毛细血管腔内有碎屑。在皮质细胞中发现了红细胞。注射毒液48小时后，观察到窗孔扩大。毛细血管基底膜中断。可见髓鞘样结构和自噬空泡。可见光滑内质网元件肿胀，内皮向光面展开。此外，还观察到高尔基体和线粒体肿胀，在某些区域形成髓鞘样结构。注射毒液144小时后，毛细血管窗孔间隙增宽。细胞切片显示光滑内质网元件肿胀并缺失。光滑内质网管状结构消失表明无类固醇生成。总之，我们认为，正如在小鼠身上观察到的那样，蜜蜂蜇伤人类的一些临床表现是由这种毒液中的毒素对肾上腺造成的损伤所决定的。
