Beharry S, Bragg P D
Department of Biochemistry, University of British Columbia, Vancouver, Canada.
Biochem Biophys Res Commun. 1992 Jan 31;182(2):697-702. doi: 10.1016/0006-291x(92)91788-r.
Beef-heart mitochondrial F1 ATPase can be induced to synthesize ATP from ADP and inorganic phosphate in 30% Me2SO. We have analyzed the adenine nucleotide content of the F1 ATPase during the time-course of ATP synthesis, in the absence of added medium nucleotide, and in the absence and presence of 10 mM inorganic phosphate. The enzyme used in these investigations was either pretreated or not pretreated with ATP to produce F1 with a defined nucleotide content and catalytic or noncatalytic nucleotide-binding site occupancy. We show that the mechanism of ATP synthesis in Me2SO involves (i) an initial rapid loss of bound nucleotide(s), this process being strongly influenced by inorganic phosphate; (ii) a rebinding of lost nucleotide; and (iii) synthesis of ATP from bound ADP and inorganic phosphate.
牛心线粒体F1 ATP酶在30%的二甲基亚砜中可被诱导由ADP和无机磷酸盐合成ATP。我们分析了在ATP合成的时间进程中，在不添加介质核苷酸的情况下，以及在不存在和存在10 mM无机磷酸盐的情况下F1 ATP酶的腺嘌呤核苷酸含量。这些研究中使用的酶要么用ATP预处理，要么不进行预处理，以产生具有确定核苷酸含量以及催化或非催化核苷酸结合位点占有率的F1。我们表明，在二甲基亚砜中ATP合成的机制涉及：（i）结合核苷酸的初始快速丢失，此过程受无机磷酸盐的强烈影响；（ii）丢失核苷酸的重新结合；以及（iii）由结合的ADP和无机磷酸盐合成ATP。
