Beharry S, Bragg P D
Department of Biochemistry, University of British Columbia, Vancouver, Canada.
FEBS Lett. 1991 Oct 21;291(2):282-4. doi: 10.1016/0014-5793(91)81302-o.
Beef-heart mitochondrial F1-ATPase contained 5 mol of inorganic phosphate bound per mol of F1, following pretreatment with ATP. A portion of the phosphate, bound most likely at a catalytic site, reacted in dimethylsulfoxide with endogenous adenine nucleotide to form ATP.
用ATP预处理后,牛心线粒体F1-ATP酶每摩尔F1含有5摩尔结合的无机磷酸。一部分磷酸(很可能结合在催化位点)在二甲基亚砜中与内源性腺嘌呤核苷酸反应形成ATP。