Reig-Armiñana J, Calatayud V, Cerveró J, García-Breijo F J, Ibars A, Sanz M J
Laboratorio de Anatomía e Histología Vegetal "Julio Iranzo", Jardín Botánico, Universitat de València, c/Quart, 80, 46008 Valencia, Spain.
Environ Pollut. 2004 Nov;132(2):321-31. doi: 10.1016/j.envpol.2004.04.006.
An open-top chamber study was conducted to investigate the tissue and cellular-level foliar effects of ozone (O3) on a Mediterranean evergreen species, the mastic plant (Pistacia lentiscus L.). Plants were exposed at three different O3 levels, and leaf samples were collected periodically from the beginning of the exposure. Although no visible foliar injury was evident, alterations of the plastids and vacuoles in the mesophyll were observed. Senescence processes were accelerated with an anomalous stacking of tannin vacuoles, and a reduction in the size and number of the chloroplasts. Overall, most of the modifications induced by O3 were consistent with previously reported observations on deciduous broadleaf species, with the exception of alterations in the cells covering the secretory channels, reported here as a new finding. Comments on the feasibility of using microscopy to validate O3 related field observations and subtle foliar injury are also given.
进行了一项开顶式气室研究，以调查臭氧（O3）对地中海常绿树种乳香黄连木（Pistacia lentiscus L.）叶片的组织和细胞水平影响。将植物暴露于三种不同的O3水平，并从暴露开始定期采集叶片样本。虽然没有明显的可见叶片损伤，但观察到叶肉中质体和液泡的变化。衰老过程加速，单宁液泡异常堆积，叶绿体大小和数量减少。总体而言，O3诱导的大多数变化与先前报道的落叶阔叶树种的观察结果一致，但覆盖分泌通道的细胞变化除外，此处报道为一项新发现。还对使用显微镜验证与O3相关的田间观察和细微叶片损伤的可行性进行了评论。
