Auttachoat Wimolnut, Chitsomboon Benjamart, Peachee Vanessa L, Guo Tai L, White Kimber L
Department of Environmental Biology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Int Immunopharmacol. 2004 Oct;4(10-11):1381-90. doi: 10.1016/j.intimp.2004.06.001.
In the previous report, we have provided evidence that Aeginetia indica Roxbert (DDD) extracts enhance T cell-mediated immune responses. The study reported here was focused on the hematological and immunological effects, including B cells, natural killer (NK) cells, macrophages and neutrophils, of the whole plant extract using water (WDDD) or ethanol (EDDD) as the solvent. The extracts were administered to female B6C3F1 mice by gavage for WDDD (10-100%) and intraperitoneally for EDDD (0.25-250 mg/kg) for 28 days. In addition to hematological evaluation, several quantitative measures and functional assays (e.g., the splenic phenotypic analysis, IgM antibody-forming cell responses, natural killer cell activity, mononuclear phagocyte system [MPS] and neutrophil activity) were employed to examine the effects of DDD extracts on the innate and humoral immunities. The results from this study demonstrated that exposure to WDDD and EDDD produced minimal changes in the activities of B cells and natural killer cells, macrophages and neutrophils. Overall, hematological parameters were not affected by exposure to WDDD or EDDD. Taken together, the enhancing effect of DDD extracts on T cells may be primarily responsible for the successful and long-time use of this traditional herbal medicine in Thailand.
在之前的报告中，我们已提供证据表明印度野菰提取物可增强T细胞介导的免疫反应。本研究聚焦于以水（WDDD）或乙醇（EDDD）为溶剂的全株植物提取物对血液学和免疫学的影响，包括对B细胞、自然杀伤（NK）细胞、巨噬细胞和中性粒细胞的影响。提取物通过灌胃给予雌性B6C3F1小鼠（WDDD为10 - 100%），通过腹腔注射给予EDDD（0.25 - 250毫克/千克），持续28天。除血液学评估外，还采用了多种定量测量方法和功能检测（如脾脏表型分析、IgM抗体形成细胞反应、自然杀伤细胞活性、单核吞噬细胞系统[MPS]和中性粒细胞活性）来研究野菰提取物对固有免疫和体液免疫的影响。本研究结果表明，接触WDDD和EDDD对B细胞、自然杀伤细胞、巨噬细胞和中性粒细胞的活性产生的变化极小。总体而言，血液学参数不受接触WDDD或EDDD的影响。综上所述，野菰提取物对T细胞的增强作用可能是这种传统草药在泰国得以成功且长期使用的主要原因。
