Auttachoat Wimolnut, Chitsomboon Benjamart, Peachee Vanessa L, Guo Tai L, White Kimber L
Department of Environmental Biology, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Int Immunopharmacol. 2004 Oct;4(10-11):1367-79. doi: 10.1016/j.intimp.2004.06.002.
Aeginetia indica Roxbert (Dok Din Daeng, DDD), a parasitic plant that grows on bamboo, is extensively used in Thai traditional medicine to treat various diseases. There have been no published studies on the pharmacological, toxicological or immunological effects of DDD, indigenous to Thailand. The study reported here was focused on the immunological effects (T cells) of the whole plant extract using water (WDDD) or ethanol (EDDD) as a solvent. The extracts were administered to female B6C3F1 mice by gavage for WDDD (10-100%) and intraperitoneally (i.p.) for EDDD (0.25-250 mg/kg) for 28 days. Only mice administrated the highest dose of EDDD exhibited an increase in absolute spleen and liver weights. Three T cell functional assays, including anti-CD3 antibody-mediated T cell proliferation, the mixed leukocyte response (MLR) and the cytotoxic T lymphocyte (CTL) response, were employed to determine the effects of DDD extracts on splenic T cell activities. Exposure to WDDD enhanced the responses in all three assays with significant changes observed in the anti-CD3 and MLR assays. Exposure to EDDD also enhanced the responses in all three assays with significant changes observed in the MLR and CTL assays. Additionally, significant increases in the MLR and anti-CD3 responses were also observed when EDDD was used to treat cells in vitro. Finally, exposure to WDDD decreased both the percentage and absolute number of regulatory CD4(+)CD25(+) T cells in the spleen, which was consistent with a significant increase in interferon-gamma (IFN-gamma) production from Con A-stimulated splenocytes. Overall, this study demonstrated that the extracts from A. indica Roxbert had a T cell stimulatory activity.
印度野菰（Dok Din Daeng，DDD）是一种生长在竹子上的寄生植物，在泰国传统医学中被广泛用于治疗各种疾病。目前尚未有关于泰国本土DDD的药理、毒理或免疫作用的公开研究。本文报道的研究聚焦于以水（WDDD）或乙醇（EDDD）为溶剂的全株提取物的免疫作用（T细胞）。提取物通过灌胃给予雌性B6C3F1小鼠用于WDDD（10 - 100%），通过腹腔注射（i.p.）用于EDDD（0.25 - 250 mg/kg），持续28天。只有给予最高剂量EDDD的小鼠脾脏和肝脏绝对重量增加。采用三种T细胞功能测定法，包括抗CD3抗体介导的T细胞增殖、混合淋巴细胞反应（MLR）和细胞毒性T淋巴细胞（CTL）反应，来确定DDD提取物对脾T细胞活性的影响。暴露于WDDD增强了所有三种测定法中的反应，在抗CD3和MLR测定法中观察到显著变化。暴露于EDDD也增强了所有三种测定法中的反应，在MLR和CTL测定法中观察到显著变化。此外，当用EDDD体外处理细胞时，MLR和抗CD3反应也显著增加。最后，暴露于WDDD降低了脾脏中调节性CD4(+)CD25(+) T细胞的百分比和绝对数量，这与伴刀豆球蛋白A刺激的脾细胞产生的干扰素 - γ（IFN - γ）显著增加一致。总体而言，本研究表明印度野菰提取物具有T细胞刺激活性。
