Peebles E D, Burnham M R, Walzem R L, Branton S L, Gerard P D
Department of Poultry Science, Mississippi State University, P.O. Box 9665, Mississippi State, MS 39762, USA.
Comp Biochem Physiol A Mol Integr Physiol. 2004 Jul;138(3):305-11. doi: 10.1016/j.cbpb.2004.04.008.
The effects of a 24-h fast on serum lipids and lipoprotein profiles in commercial laying hens were investigated. Blood was analyzed at 34 and 46 weeks of age from Single Comb White Leghorn hens that had been either fed ad libitum or had been fasted for 24 h prior to collection. At 12 weeks, birds were divided into 16 biological isolation units, with 8 replicate units assigned to each treatment group. Four birds out of 10 in each unit were tagged for bleeding. Parameters evaluated included total serum cholesterol and triglycerides, mean diameters of very low density lipoproteins (VLDLs) for the 10th, 50th, and 90th percentiles of serum total VLDL, mean total population VLDL particle diameter (MPD), and percentage serum cholesterol recovered in VLDL, low density lipoprotein (LDL), and high density lipoprotein (HDL) fractions. Fasting led to decreases in total serum cholesterol and triglycerides, and a decrease in mean serum VLDL particle diameter in the 90th population percentile. At Week 34, percentage serum cholesterol recovered from LDL was increased, whereas percentage serum cholesterol recovered from HDL was decreased due to fasting. At Week 46, MPD and percentage serum cholesterol recovered from VLDL were decreased, whereas percentage serum cholesterol recovered from HDL was increased due to fasting. It was concluded that a 24-h fast decreased serum lipids (cholesterol and triglycerides) and the size of VLDL particles in the 90th population percentile in commercial laying hens. Furthermore, bird age influenced the effects of a 24-h fast on MPD and the redistribution of serum cholesterol among VLDL, LDL, and HDL particles.
研究了24小时禁食对商品蛋鸡血清脂质和脂蛋白谱的影响。在34周龄和46周龄时，对单冠白来航蛋鸡的血液进行分析，这些蛋鸡要么自由采食，要么在采血前禁食24小时。在12周龄时，将鸡分为16个生物隔离单元，每个处理组分配8个重复单元。每个单元的10只鸡中有4只被标记用于采血。评估的参数包括血清总胆固醇和甘油三酯、血清总极低密度脂蛋白（VLDL）第10、50和90百分位数的极低密度脂蛋白平均直径、总群体VLDL颗粒平均直径（MPD）以及VLDL、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）组分中回收的血清胆固醇百分比。禁食导致血清总胆固醇和甘油三酯降低，以及第90群体百分位数的血清VLDL颗粒平均直径减小。在34周时，禁食导致从LDL中回收的血清胆固醇百分比增加，而从HDL中回收的血清胆固醇百分比降低。在46周时，MPD和从VLDL中回收的血清胆固醇百分比降低，而禁食导致从HDL中回收的血清胆固醇百分比增加。得出的结论是，24小时禁食降低了商品蛋鸡的血清脂质（胆固醇和甘油三酯）以及第90群体百分位数的VLDL颗粒大小。此外，鸡的年龄影响了24小时禁食对MPD以及血清胆固醇在VLDL、LDL和HDL颗粒之间重新分布的影响。
