Tibell A, Ringdén O, Sundberg B
Department of Transplantation Surgery, Huddinge Hospital, Sweden.
Scand J Immunol. 1992 Feb;35(2):231-6. doi: 10.1111/j.1365-3083.1992.tb02855.x.
We have recently shown in a rat model that the acute decrease in glomerular filtration rate caused by intravenous Sandimmun can be avoided if Cyclosporine A (CyA) is given in a fat emulsion carrier instead. The present study was undertaken to determine the immunosuppressive effect in vitro of this new formulation. The inhibitory effect on the lymphocyte responsiveness of CyA in fat emulsion carrier was compared with that of CyA dissolved in alcohol, using mitogens as well as alloantigens as stimuli. In the mixed lymphocyte culture and cell-mediated lympholysis tests and in the interleukin-2 assay, both formulations seemed equally effective. CyA in fat emulsion was somewhat less effective than CyA in alcohol in the mitogen tests. In clinical practice the fat emulsion would be rapidly metabolized, and to further evaluate our new formulation, studies in experimental organ transplantation are needed.
我们最近在大鼠模型中表明，如果用脂肪乳剂载体给予环孢素A（CyA），而非静脉注射山地明（Sandimmun），则可避免由其导致的肾小球滤过率急性下降。本研究旨在确定这种新制剂在体外的免疫抑制作用。使用丝裂原和同种异体抗原作为刺激物，比较了脂肪乳剂载体中环孢素A对淋巴细胞反应性的抑制作用与溶解于酒精中的环孢素A的抑制作用。在混合淋巴细胞培养和细胞介导的淋巴细胞溶解试验以及白细胞介素-2测定中，两种制剂似乎同样有效。在丝裂原试验中，脂肪乳剂中的环孢素A比酒精中的环孢素A效果稍差。在临床实践中，脂肪乳剂会迅速代谢，为了进一步评估我们的新制剂，需要在实验性器官移植中进行研究。
