四膜虫中特定结合的小RNA具有预测的参与基因组重排的扫描（scn）RNA的特性。
Conjugation-specific small RNAs in Tetrahymena have predicted properties of scan (scn) RNAs involved in genome rearrangement.
作者信息
Mochizuki Kazufumi, Gorovsky Martin A
机构信息
Department of Biology, University of Rochester, Rochester, New York 14627, USA.
出版信息
Genes Dev. 2004 Sep 1;18(17):2068-73. doi: 10.1101/gad.1219904. Epub 2004 Aug 16.
Abstract
We proposed a scan-RNA model for genome rearrangement based on finding small RNAs that hybridized preferentially to micronuclear-specific sequences and on the properties of Twi1p, a PPD protein required for both sequence elimination and small RNA accumulation in Tetrahymena. Here we show that Twi1p interacts with the small RNAs in both the old and the developing macronucleus, and is required for their stability. We show that the specificity of the small RNAs for micronuclear-limited sequences increases during conjugation. These results indicate that the small RNAs observed in conjugating cells have the properties predicted for scan RNAs.
摘要
我们基于发现优先与微核特异性序列杂交的小RNA以及Twi1p（四膜虫中序列消除和小RNA积累所需的一种PPD蛋白）的特性，提出了一种用于基因组重排的扫描RNA模型。在此我们表明，Twi1p在旧的和发育中的大核中均与小RNA相互作用，并且是它们稳定性所必需的。我们表明，在接合过程中，小RNA对微核有限序列的特异性增加。这些结果表明，在接合细胞中观察到的小RNA具有扫描RNA所预测的特性。
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