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白塞病中T细胞调节性细胞因子IL-10和IL-12的全身水平；可溶性肿瘤坏死因子受体-75作为疾病活动的生物标志物

Systemic levels of the T cell regulatory cytokines IL-10 and IL-12 in Bechçet's disease; soluble TNFR-75 as a biological marker of disease activity.

作者信息

Turan B, Gallati H, Erdi H, Gürler A, Michel B A, Villiger P M

机构信息

Department of Rheumatology, University Hospital, Zürich, Switzerland.

出版信息

J Rheumatol. 1997 Jan;24(1):128-32.

PMID:9002023

Abstract

OBJECTIVE

To analyze the levels of the T cell regulatory cytokines interleukin 10 (IL-10) and IL-12 in plasma of patients with Behçet's disease (BD), and to assess the value of cytokines and cytokine antagonists as biological markers of disease activity.

METHODS

Sera/plasma of 66 consecutive outpatients with established diagnosis of BD were analyzed for the presence of IL-2R, IL-6, tumor necrosis factor-alpha (TNF-alpha), soluble (s) TNF receptor (R)-55, sTNFR-75, IL-10, and IL-12 using immunological methods. Additional laboratory measurements included erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP). Data from the history and clinical examination were recorded to correlate cytokine levels with clinical markers of disease activity.

RESULTS

18 patients had inactive (Group I), 36 had mildly active (Group II), and 12 patients had active BD (Group III). IL-10 was elevated in 42 plasma samples (64%). The percentage of samples containing IL-10 and the median levels of IL-10 of the 3 patient groups did not differ significantly. IL-12 was detectable in plasma of 9 patients: One from Group I (5%), 3 from Group II (8%), and 5 from Group III (41%). IL-12 correlated with disease activity (difference between Groups I and III, p = 0.02, between Groups II and III, p = 0.008). ESR in patients with active disease and mildly active disease was significantly higher than values in patients with inactive disease (p = 0.03, p = 0.02, respectively), while median CRP levels were significantly different between Group I and Group III only (p = 0.006). sTNFR-75 levels were significantly different between Groups II and III (p = 0.003) and between Groups I and III (p = 0.008).

CONCLUSION

The elevation of plasma IL-10 in the majority of patients and the correlation of IL-12 plasma levels with disease activity suggest a pathogenic role of a TH1-type immune response in active disease. In addition, the correlation of sTNFR-75 levels with disease activity indicates that sTNFR-75 may serve as a biological marker of disease activity in BD.

摘要

目的

分析白塞病（BD）患者血浆中T细胞调节细胞因子白细胞介素10（IL-10）和IL-12的水平，并评估细胞因子及细胞因子拮抗剂作为疾病活动生物标志物的价值。

方法

采用免疫学方法分析66例确诊为BD的门诊连续患者的血清/血浆中IL-2R、IL-6、肿瘤坏死因子-α（TNF-α）、可溶性（s）TNF受体（R）-55、sTNFR-75、IL-10和IL-12的存在情况。其他实验室检测指标包括红细胞沉降率（ESR）和C反应蛋白（CRP）。记录病史和临床检查数据，以将细胞因子水平与疾病活动的临床指标相关联。

结果

18例患者病情不活动（I组），36例患者病情轻度活动（II组），12例患者病情活动（III组）。42份血浆样本（64%）中IL-10升高。3组患者中含有IL-10的样本百分比及IL-10的中位数水平无显著差异。9例患者血浆中可检测到IL-12：I组1例（5%），II组3例（8%），III组5例（41%）。IL-12与疾病活动相关（I组和III组之间差异，p = 0.02；II组和III组之间差异，p = 0.008）。病情活动和轻度活动患者的ESR显著高于病情不活动患者（分别为p = 0.03，p = 0.02），而仅I组和III组之间的CRP中位数水平有显著差异（p = 0.006）。II组和III组之间以及I组和III组之间的sTNFR-75水平有显著差异（p = 0.003，p = 0.008）。