Singh Kaman, Shukla A K
Department of Chemistry, Government Autonomous Postgraduate College, Tikamgarh.
Indian J Environ Health. 2003 Jul;45(3):203-8.
A selective and sensitive method is described for the microdetermination of phosphate in water and wastewater employing a new reagent system. The method is based on the reaction between ammonium molybdate, PVA (stabilizer) and malachite green (lambdamax 600nm). Beer's law is obeyed up to 10ppm. The use of PVA as a substitute for Tween 20 makes more soluble the malachite green-phosphomolybdic acid complex; it becomes more sensitive and less of reagents are needed to obtain the same sensitivity as when Tween 20 is used as a stabilizer. It offers advantages like reliability, non-interference by silicates and reproducible in addition to its simplicity, instant colour development and allows economy in reagents such as ammonium molybdate and malachite green. It's cost is less than half of the Tween 20 method. This method is more suited for the measurements for the range of 0.01 to 10 mg p/L.
描述了一种采用新型试剂系统对水和废水中的磷酸盐进行微量测定的选择性和灵敏方法。该方法基于钼酸铵、聚乙烯醇（稳定剂）和孔雀石绿（最大吸收波长600nm）之间的反应。在高达10ppm的范围内符合比尔定律。使用聚乙烯醇替代吐温20可使孔雀石绿 - 磷钼酸络合物更易溶解；它变得更灵敏，并且与使用吐温20作为稳定剂时相比，获得相同灵敏度所需的试剂更少。除了简单、显色快以及能节省钼酸铵和孔雀石绿等试剂外，它还具有可靠性、不受硅酸盐干扰和可重现等优点。其成本不到吐温20方法的一半。该方法更适合于0.01至10mg p/L范围内的测量。
