School of Chemistry and Chemical Engineering, University of South China, Hengyang Hunan 421001, China.
J Hazard Mater. 2010 Mar 15;175(1-3):883-8. doi: 10.1016/j.jhazmat.2009.10.092. Epub 2009 Oct 31.
In this work, a new method has been proposed to simultaneously determine the trace amount of malachite green and crystal violet from aqueous solution by spectrophotometry after cloud point extraction (CPE) using partial least squares regression. The optimal extraction and operating conditions, such as pH, reagents concentration and effect of time and temperature, and so on, have been investigated using the non-ionic surfactant Triton X-114. The maximum absorption wavelength for malachite green and crystal violet is 624 and 579 nm, respectively; linearity is obeyed in the range of 9.9-800 and 16-1000 ng mL(-1) with detection limit of 2.9 and 4.8 ng mL(-1), and the root mean square error of prediction (RMSEP) are 0.0197 and 0.0343, respectively. The proposed method has been applied successfully to simultaneously determine the trace amount of malachite green and crystal violet in real matrix samples with the recoveries of 92.45-102.5%.
在这项工作中，提出了一种新的方法，通过使用浊点萃取（CPE）后，使用偏最小二乘回归分光光度法同时测定水溶液中孔雀石绿和结晶紫的痕量。使用非离子表面活性剂 Triton X-114 研究了最佳的萃取和操作条件，如 pH 值、试剂浓度以及时间和温度等的影响。孔雀石绿和结晶紫的最大吸收波长分别为 624nm 和 579nm；在 9.9-800ng/mL 和 16-1000ng/mL 范围内呈线性，检测限分别为 2.9ng/mL 和 4.8ng/mL，预测均方根误差（RMSEP）分别为 0.0197 和 0.0343。该方法已成功应用于实际基质样品中同时测定痕量孔雀石绿和结晶紫，回收率为 92.45-102.5%。
