人巨细胞病毒对体外巨核细胞集落形成单位形成的影响。

The effect of human cytomegalovirus on the formation of CFU-MK in vitro.

作者信息

Yao Junxia, Song Sanjun, Hu Lihua

机构信息

Institute of Heamatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

出版信息

J Huazhong Univ Sci Technolog Med Sci. 2004;24(2):134-7. doi: 10.1007/BF02885411.

DOI:10.1007/BF02885411

PMID:15315162

Abstract

To investigate the mechanism and the suppressive effect of human cytomegalovirus (HCMV) on colony forming unit-megakaryocyte (CFU-MK), semi-solid culture system was used to observe the effect of HCMV AD169 strain on CFU-MK's growth of 18 cord blood samples. HCMV DNA and immediate early (IE) protein mRNA in CFU-MK was detected by PCR and reverse transcription-polymerase chain reaction (RT-PCR). Our results showed that HCMV AD169 significantly suppressed the formation of CFU-MK in vitro. Compared with the mock group, the CFU-MK colonies decreased by 21.6%, 33.8% and 46.3%, respectively, in all the 3 infected groups (P<0.05), suggesting the suppression and the titer of the virus was dose-dependent. Both HCMV DNA and the expression of HCMV IE protein mRNA were positively detected in the colony cells of viral infected group. It is concluded that HCMV AD169 strain could inhibit the differentiation and proliferation of CFU-MK by directly infecting their progenitors. There was early transcription of HCMV IE protein in CFU-MK infected by virus.

摘要

为研究人巨细胞病毒（HCMV）对巨核细胞集落形成单位（CFU-MK）的作用机制及抑制效应，采用半固体培养系统观察HCMV AD169株对18份脐血样本中CFU-MK生长的影响。通过聚合酶链反应（PCR）和逆转录-聚合酶链反应（RT-PCR）检测CFU-MK中的HCMV DNA和即刻早期（IE）蛋白mRNA。结果显示，HCMV AD169在体外显著抑制CFU-MK的形成。与 mock组相比，3个感染组的CFU-MK集落分别减少了21.6%、33.8%和46.3%（P<0.05），提示病毒的抑制作用和滴度呈剂量依赖性。在病毒感染组的集落细胞中均阳性检测到HCMV DNA和HCMV IE蛋白mRNA的表达。结论：HCMV AD169株可通过直接感染CFU-MK的祖细胞来抑制其分化和增殖。病毒感染的CFU-MK中有HCMV IE蛋白的早期转录。