Wu Zhigang, Rodgers Ryan P, Marshall Alan G
Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, Florida 32310-4005, USA.
J Agric Food Chem. 2004 Aug 25;52(17):5322-8. doi: 10.1021/jf049596q.
Adulteration of vegetable oil is of concern for both commercial and health reasons. Compositional based fingerprints can potentially reveal both the oil source and its possible adulteration. Here, electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) resolves and identifies literally thousands of distinct chemical components of commercial canola, olive, and soybean oils, without extraction or other wet chemical separation pretreatment. In negative-ion ESI FT-ICR MS, the acidic components of soybean oil are easily distinguished from those of canola and olive oil based on relative abundances of C(18) fatty acids, whereas olive oil differs from canola and soybean oil based on relative abundances of tocopherols. In positive-ion ESI FT-ICR MS, the three oils are readily distinguished according to the relative abundances of di- and triacylglycerols with various numbers of double bonds in the fatty acid chains. We demonstrate the detection of soybean oil as an adulterant of olive oil, based on relative abundances of members of each of several chemical families. We suggest that the detailed chemical compositions of vegetable oils can be used to characterize them and to detect and identify adulterants.
出于商业和健康原因，植物油掺假备受关注。基于成分的指纹图谱有可能揭示油的来源及其可能的掺假情况。在此，电喷雾电离（ESI）傅里叶变换离子回旋共振质谱（FT-ICR MS）无需萃取或其他湿化学分离预处理，就能解析并识别商用油菜籽油、橄榄油和大豆油中数千种不同的化学成分。在负离子ESI FT-ICR MS中，基于C(18)脂肪酸的相对丰度，大豆油的酸性成分很容易与油菜籽油和橄榄油的区分开来，而基于生育酚的相对丰度，橄榄油与油菜籽油和大豆油不同。在正离子ESI FT-ICR MS中，根据脂肪酸链中双键数量不同的二酰基甘油和三酰基甘油的相对丰度，这三种油很容易区分开来。我们基于几个化学家族中各成员的相对丰度，证明了能检测出大豆油作为橄榄油的掺假物。我们认为植物油的详细化学成分可用于对其进行表征以及检测和识别掺假物。
