Modolo F, Martins M T, Loducca S V L, de Araújo V C
Department of Oral Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Oral Dis. 2004 Sep;10(5):277-82. doi: 10.1111/j.1601-0825.2004.01028.x.
To analyze integrin expression and distribution in different histological types of ameloblastoma, compared with dental germ, dental lamina and adult lining epithelium.
Three-micrometer sections from paraffin-embedded specimens were evaluated employing a streptavidin-biotin immunohistochemical method and anti-integrin alpha2, alpha3, alpha5, alphav, beta1, beta3 and beta4 antibodies.
All integrins were present in all specimens, exhibiting different patterns. In follicular ameloblastoma, the integrin staining was stronger in the periphery while integrin alpha2 was not present in the central cells. Acanthomatous ameloblastoma showed a similar pattern, with positive staining for integrins alpha3, alpha5, alphav, beta1 and beta4 in the metaplastic cells. In the unicystic, integrin staining was uniform except for integrins alpha5 and beta3 which showed weaker staining in the upper layers. In the plexiform ameloblastoma, dental germ and lamina integrin staining was uniform. In the adult lining epithelium, staining for integrins alpha2, alpha5 and beta4 was confined to the basal layer, while integrins alphav and beta3 were present in the basal and parabasal, with integrins alpha3 and beta1 in the upper layers.
Acanthomatous, follicular and unicystic ameloblastomas showed integrin staining patterns similar to the adult lining epithelium while the plexiform ameloblastoma was similar to the dental germ and lamina.
分析整合素在不同组织学类型成釉细胞瘤中的表达及分布，并与牙胚、牙板和成人衬里上皮进行比较。
采用链霉亲和素-生物素免疫组化方法及抗整合素α2、α3、α5、αv、β1、β3和β4抗体，对石蜡包埋标本的3微米切片进行评估。
所有标本中均存在所有整合素，呈现不同模式。在滤泡型成釉细胞瘤中，整合素染色在周边较强，而中央细胞中不存在整合素α2。棘皮瘤型成釉细胞瘤表现出类似模式，化生细胞中整合素α3、α5、αv、β1和β4呈阳性染色。在单囊型中，除整合素α5和β3在上层染色较弱外，整合素染色均匀。在丛状成釉细胞瘤、牙胚和牙板中，整合素染色均匀。在成人衬里上皮中，整合素α2、α5和β4的染色局限于基底层，而整合素αv和β3存在于基底层和副基底层，整合素α3和β1存在于上层。
棘皮瘤型、滤泡型和单囊型成釉细胞瘤的整合素染色模式与成人衬里上皮相似，而丛状成釉细胞瘤与牙胚和牙板相似。
